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1bus

From Proteopedia

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(New page: 200px<br /><applet load="1bus" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bus" /> '''SOLUTION CONFORMATION OF PROTEINASE INHIBITO...)
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[[Image:1bus.gif|left|200px]]<br /><applet load="1bus" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bus" />
 
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'''SOLUTION CONFORMATION OF PROTEINASE INHIBITOR IIA FROM BULL SEMINAL PLASMA BY 1H NUCLEAR MAGNETIC RESONANCE AND DISTANCE GEOMETRY'''<br />
 
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==Overview==
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==SOLUTION CONFORMATION OF PROTEINASE INHIBITOR IIA FROM BULL SEMINAL PLASMA BY 1H NUCLEAR MAGNETIC RESONANCE AND DISTANCE GEOMETRY==
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A determination of the solution conformation of the proteinase inhibitor, IIA from bull seminal plasma (BUSI IIA) is described. Two-dimensional, nuclear Overhauser enhancement spectroscopy (NOESY) was used to obtain a, list of 202 distance constraints between individually assigned hydrogen, atoms of the polypeptide chain, to identify the positions of the three, disulfide bridges, and to locate the single cis peptide bond., Supplementary geometric constraints were derived from the vicinal, spin-spin couplings and the locations of certain hydrogen bonds, as, determined by nuclear magnetic resonance (n.m.r.). Using a new distance, geometry program (DISGEO) which is capable of computing all-atom, structures for proteins the size of BUSI IIA, five conformers were, computed from the NOE distance constraints alone, and another five were, computed with the supplementary constraints included. Comparison of the, different structures computed from the n.m.r. data among themselves and, with the crystal structures of two homologous proteins shows that the, global features of the conformation of BUSI IIA (i.e. the overall, dimensions of the molecule and the threading of the polypeptide chain), were well-defined by the available n.m.r. data. In the Appendix, we, describe a preliminary energy refinement of the structure, which showed, that the constraints derived from the n.m.r. data are compatible with a, low energy spatial structure.
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<StructureSection load='1bus' size='340' side='right'caption='[[1bus]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bus]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BUS FirstGlance]. <br>
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1BUS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BUS OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bus OCA], [https://pdbe.org/1bus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bus RCSB], [https://www.ebi.ac.uk/pdbsum/1bus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bus ProSAT]</span></td></tr>
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Solution conformation of proteinase inhibitor IIA from bull seminal plasma by 1H nuclear magnetic resonance and distance geometry., Williamson MP, Havel TF, Wuthrich K, J Mol Biol. 1985 Mar 20;182(2):295-315. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3839023 3839023]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ISK6_BOVIN ISK6_BOVIN] Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8. Inhibits acrosin, trypsin, and chymotrypsin.[UniProtKB:Q6UWN8]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bu/1bus_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bus ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Guntert, P.]]
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[[Category: Guntert P]]
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[[Category: Havel, T.F.]]
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[[Category: Havel TF]]
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[[Category: Williamson, M.P.]]
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[[Category: Williamson MP]]
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[[Category: Wuthrich, K.]]
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[[Category: Wuthrich K]]
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[[Category: proteinase inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:56:26 2007''
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Current revision

SOLUTION CONFORMATION OF PROTEINASE INHIBITOR IIA FROM BULL SEMINAL PLASMA BY 1H NUCLEAR MAGNETIC RESONANCE AND DISTANCE GEOMETRY

PDB ID 1bus

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