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1r0c
From Proteopedia
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'''Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme''' | '''Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme''' | ||
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[[Category: Huang, J.]] | [[Category: Huang, J.]] | ||
[[Category: Lipscomb, W N.]] | [[Category: Lipscomb, W N.]] | ||
| - | [[Category: | + | [[Category: Aspartate carbamoyltransferase]] |
| - | [[Category: | + | [[Category: Aspartate transcarbamylase]] |
| - | [[Category: | + | [[Category: Atcase-products complex]] |
| - | [[Category: | + | [[Category: Phosphate]] |
| - | [[Category: | + | [[Category: Product]] |
| - | [[Category: | + | [[Category: T state]] |
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Revision as of 03:55, 3 May 2008
Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme
Overview
The structure of aspartate transcarbamylase of Escherichia coli ligated to products (phosphate and N-carbamyl-l-aspartate) has been determined at 2.37 A resolution (R-factor = 0.23, R(free) = 0.27). Results might indicate a product release mode, rather than close analogues to the transition state like those found in our earlier studies of other ligands (N-phosphonacetyl-L-aspartate, carbamyl phosphate plus malonate, phosphonoacetamide plus malonate, or citrate plus phosphate). Ordered product release, first carbamylaspartate (CLA) and then phosphate, might be facilitated by a 4 A movement of phosphate from the substrate-analogue position to the product (phosphate) binding position, and by a somewhat similar release movement of the other product (CLA) relative to its analogue (citrate). This movement is consistent with earlier studies of binding of either pyrophosphate or phosphate alone [Honzatko, R. B., and Lipscomb, W. N. (1982) J. Mol. Biol. 160, 265-286].
About this Structure
1R0C is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Products in the T-state of aspartate transcarbamylase: crystal structure of the phosphate and N-carbamyl-L-aspartate ligated enzyme., Huang J, Lipscomb WN, Biochemistry. 2004 Jun 1;43(21):6422-6. PMID:15157076 Page seeded by OCA on Sat May 3 06:55:08 2008
