This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1cch

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1cch" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cch" /> '''THE SOLUTION CONFORMATION OF CYTOCHROME C-55...)
Current revision (15:38, 13 March 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1cch.gif|left|200px]]<br /><applet load="1cch" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1cch" />
 
-
'''THE SOLUTION CONFORMATION OF CYTOCHROME C-551 FROM P.STUTZERI ZOBELL DETERMINED BY NMR+'''<br />
 
-
==Overview==
+
==THE SOLUTION CONFORMATION OF CYTOCHROME C-551 FROM P.STUTZERI ZOBELL DETERMINED BY NMR+==
-
1H NMR spectroscopy and solution structure computations have been used to, examine ferrocytochrome c-551 from Pseudomonas stutzeri (ATCC 17588)., Resonance assignments are proposed for all main-chain and most side-chain, protons. Distance constraints were determined on the basis of nuclear, Overhauser enhancements between pairs of protons. Dihedral angle, constraints were determined from estimates of scaler coupling constants., Twenty-four structures were calculated by distance geometry and refined by, energy minimization and simulated annealing on the basis of 1033, interproton distance and 57 torsion angle constraints. Both the main-chain, and side-chain atoms are well defined except for a loop region around, residues 34-40, the first two residues at the N-terminus and the last two, at the C-terminus, and some side chains located on the molecular surface., The average root mean squared deviation in position for equivalent atoms, between the 24 individual structures and the mean structure obtained by, averaging their coordinates is 0.54 +/- 0.08 A for the main-chain atoms, and 0.97 +/- 0.09 A for all non-hydrogen atoms of residues 3-80 plus the, heme group. These structures were compared to the X-ray crystallographic, structure of an analogous protein, cytochrome c-551 from Pseudomonas, aeruginosa [Matsuura, Takano, &amp; Dickerson (1982) J. Mol. Biol. 156, 389-409). The main-chain folding patterns are very consistent, but there, are some differences. The largest difference is in a surface loop segment, from residues 34 to 40.
+
<StructureSection load='1cch' size='340' side='right'caption='[[1cch]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1cch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CCH FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cch OCA], [https://pdbe.org/1cch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cch RCSB], [https://www.ebi.ac.uk/pdbsum/1cch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cch ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CY551_STUST CY551_STUST] Electron donor for cytochrome cd1 in nitrite and nitrate respiration.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/1cch_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cch ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1CCH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CCH OCA].
+
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Investigation of the solution conformation of cytochrome c-551 from Pseudomonas stutzeri., Cai M, Bradford EG, Timkovich R, Biochemistry. 1992 Sep 15;31(36):8603-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1327105 1327105]
+
[[Category: Large Structures]]
[[Category: Pseudomonas stutzeri]]
[[Category: Pseudomonas stutzeri]]
-
[[Category: Single protein]]
+
[[Category: Cai M]]
-
[[Category: Cai, M.]]
+
[[Category: Timkovich R]]
-
[[Category: Timkovich, R.]]
+
-
[[Category: HEM]]
+
-
[[Category: electron transport]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:20:54 2007''
+

Current revision

THE SOLUTION CONFORMATION OF CYTOCHROME C-551 FROM P.STUTZERI ZOBELL DETERMINED BY NMR+

PDB ID 1cch

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cch"
Personal tools