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1cdt

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(New page: 200px<br /><applet load="1cdt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cdt, resolution 2.5&Aring;" /> '''CARDIOTOXIN V4/II FRO...)
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[[Image:1cdt.jpg|left|200px]]<br /><applet load="1cdt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cdt, resolution 2.5&Aring;" />
 
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'''CARDIOTOXIN V4/II FROM NAJA MOSSAMBICA MOSSAMBICA: THE REFINED CRYSTAL STRUCTURE'''<br />
 
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==Overview==
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==CARDIOTOXIN V4/II FROM NAJA MOSSAMBICA MOSSAMBICA: THE REFINED CRYSTAL STRUCTURE==
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The crystal structure of cardiotoxin VII4 from Naja mossambica mossambica, was refined to 2.5 A resolution. Fifty ordered solvent sites were, localized and included in the refinement. The final R factor is 0.197, (lambda/(2sin theta) less than 5 A; F greater than 3 sigma). The, three-dimensional structure is characterized by two beta-sheets. Of, particular interest is the two-stranded beta-sheet in the N-terminal, region. This shows a large right-handed twist and, though strongly, connected to the core of the molecule, and in particular to the C-terminal, end, protrudes out of the bulk of the molecule. The segment of four amino, acid residues connecting the two strands of this sheet is particularly, exposed. It contains an invariant proline residue that has probably an, important structural role, and is completely hydrophobic. Two other, conserved hydrophobic zones were identified; the largest extends over the, second and third loops, on one side only of the molecule. All side-chains, of invariant hydrophobic character (except proline residues) belong to one, of these three zones. Also discussed are the dimeric assembly and the, rather loose packing in the crystal. The three-dimensional structure is, compared with that of short and long alpha-neurotoxins. Comparison with, two-dimensional nuclear magnetic resonance results on the 68% homologous, cardiotoxin CT X IIb shows an excellent overall agreement. A few, differences are probably genuine.
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<StructureSection load='1cdt' size='340' side='right'caption='[[1cdt]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cdt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_mossambica Naja mossambica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CDT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cdt OCA], [https://pdbe.org/1cdt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cdt RCSB], [https://www.ebi.ac.uk/pdbsum/1cdt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cdt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/3SA4_NAJMO 3SA4_NAJMO] Shows cytolytic activity on many different cells by forming pore in lipid membranes. In vivo, increases heart rate or kills the animal by cardiac arrest. In addition, it binds to heparin with high affinity, interacts with Kv channel-interacting protein 1 (KCNIP1) in a calcium-independent manner, and binds to integrin alpha-V/beta-3 (ITGAV/ITGB3) with moderate affinity.[UniProtKB:P60301][UniProtKB:P60304]<ref>PMID:8182052</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/1cdt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cdt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CDT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_mossambica Naja mossambica] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CDT OCA].
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*[[Cardiotoxin 3D structures|Cardiotoxin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Cardiotoxin VII4 from Naja mossambica mossambica. The refined crystal structure., Rees B, Bilwes A, Samama JP, Moras D, J Mol Biol. 1990 Jul 5;214(1):281-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2370666 2370666]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Naja mossambica]]
[[Category: Naja mossambica]]
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[[Category: Single protein]]
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[[Category: Bilwes A]]
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[[Category: Bilwes, A.]]
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[[Category: Moras D]]
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[[Category: Moras, D.]]
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[[Category: Rees B]]
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[[Category: Rees, B.]]
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[[Category: Samama JP]]
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[[Category: Samama, J.P.]]
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[[Category: PO4]]
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[[Category: cytotoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:22:27 2007''
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Current revision

CARDIOTOXIN V4/II FROM NAJA MOSSAMBICA MOSSAMBICA: THE REFINED CRYSTAL STRUCTURE

PDB ID 1cdt

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