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1d4c

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(New page: 200px<br /><applet load="1d4c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d4c, resolution 2.9&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1d4c.jpg|left|200px]]<br /><applet load="1d4c" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1d4c, resolution 2.9&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1==
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Fumarate respiration is one of the most widespread types of anaerobic, respiration. The soluble fumarate reductase of Shewanella putrefaciens, MR-1 is a periplasmic tetraheme flavocytochrome c. The crystal structures, of the enzyme were solved to 2.9 A for the uncomplexed form and to 2.8 A, and 2.5 A for the fumarate and the succinate-bound protein, respectively., The structures reveal a flexible capping domain linked to the FAD-binding, domain. A catalytic mechanism for fumarate reduction based on the, structure of the complexed protein is proposed. The mechanism for the, reverse reaction is a model for the homologous succinate dehydrogenase, (complex II) of the respiratory chain. In flavocytochrome c fumarate, reductase, all redox centers are in van der Waals contact with one, another, thus providing an efficient conduit of electrons from the hemes, via the FAD to fumarate.
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<StructureSection load='1d4c' size='340' side='right'caption='[[1d4c]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1d4c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis Shewanella oneidensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D4C FirstGlance]. <br>
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1D4C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shewanella_putrefaciens Shewanella putrefaciens] with SO4, HEM and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D4C OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d4c OCA], [https://pdbe.org/1d4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d4c RCSB], [https://www.ebi.ac.uk/pdbsum/1d4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d4c ProSAT]</span></td></tr>
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Structure and mechanism of the flavocytochrome c fumarate reductase of Shewanella putrefaciens MR-1., Leys D, Tsapin AS, Nealson KH, Meyer TE, Cusanovich MA, Van Beeumen JJ, Nat Struct Biol. 1999 Dec;6(12):1113-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10581551 10581551]
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</table>
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[[Category: Shewanella putrefaciens]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/FRDA_SHEON FRDA_SHEON] Catalyzes fumarate reduction using artificial electron donors such as methyl viologen. The physiological reductant is unknown, but evidence indicates that flavocytochrome c participates in electron transfer from formate to fumarate and possibly also to trimethylamine oxide (TMAO). This enzyme is essentially unidirectional (By similarity).
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[[Category: Succinate dehydrogenase]]
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== Evolutionary Conservation ==
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[[Category: Beeumen, J.J.Van.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Cusanovich, M.A.]]
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Check<jmol>
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[[Category: Leys, D.]]
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<jmolCheckbox>
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[[Category: Meyer, T.E.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d4/1d4c_consurf.spt"</scriptWhenChecked>
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[[Category: Tsapin, A.S.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: FAD]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: HEM]]
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</jmolCheckbox>
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[[Category: SO4]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d4c ConSurf].
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[[Category: tetraheme flavocytochrome c]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:59:55 2007''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Shewanella oneidensis]]
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[[Category: Cusanovich MA]]
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[[Category: Leys D]]
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[[Category: Meyer TE]]
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[[Category: Tsapin AS]]
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[[Category: Van Beeumen JJ]]

Current revision

CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1

PDB ID 1d4c

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