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1r12
From Proteopedia
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[[Image:1r12.jpg|left|200px]] | [[Image:1r12.jpg|left|200px]] | ||
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'''Native Aplysia ADP ribosyl cyclase''' | '''Native Aplysia ADP ribosyl cyclase''' | ||
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ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate., Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q, Structure. 2004 Mar;12(3):477-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016363 15016363] | ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate., Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q, Structure. 2004 Mar;12(3):477-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016363 15016363] | ||
[[Category: Aplysia californica]] | [[Category: Aplysia californica]] | ||
| - | [[Category: NAD(+) nucleosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Graeff, R.]] | [[Category: Graeff, R.]] | ||
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[[Category: Szebenyi, D M.E.]] | [[Category: Szebenyi, D M.E.]] | ||
[[Category: Thiel, D J.]] | [[Category: Thiel, D J.]] | ||
| - | [[Category: | + | [[Category: Adp-ribosyl cyclase]] |
| - | [[Category: | + | [[Category: Ca2+ signalling]] |
| - | [[Category: | + | [[Category: Cyclic adp-ribose]] |
| - | [[Category: | + | [[Category: Naadp]] |
| - | [[Category: | + | [[Category: X-ray crystallography]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:56:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:56, 3 May 2008
Native Aplysia ADP ribosyl cyclase
Overview
ADP-ribosyl cyclase catalyzes the elimination of nicotinamide from NAD and cyclization to cADPR, a known second messenger in cellular calcium signaling pathways. We have determined to 2.0 A resolution the structure of Aplysia cyclase with ribose-5-phosphate bound covalently at C3' and with the base exchange substrate (BES), pyridylcarbinol, bound to the active site. In addition, further refinement at 2.4 A resolution of the structure of nicotinamide-bound cyclase, which was previously reported, reveals that ribose-5-phosphate is also covalently bound in this structure, and a second nicotinamide site was identified. The structures of native and mutant Glu179Ala cyclase were also solved to 1.7 and 2.0 A respectively. It is proposed that the second nicotinamide site serves to promote cyclization by clearing the active site of the nicotinamide byproduct. Moreover, a ribosylation mechanism can be proposed in which the cyclization reaction proceeds through a covalently bound intermediate.
About this Structure
1R12 is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.
Reference
ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate., Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q, Structure. 2004 Mar;12(3):477-86. PMID:15016363 Page seeded by OCA on Sat May 3 06:56:42 2008
