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3qc9

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==Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg==
==Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg==
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<StructureSection load='3qc9' size='340' side='right' caption='[[3qc9]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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<StructureSection load='3qc9' size='340' side='right'caption='[[3qc9]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qc9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QC9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qc9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QC9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRK1, RHOK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Rhodopsin_kinase Rhodopsin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.14 2.7.11.14] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qc9 OCA], [https://pdbe.org/3qc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qc9 RCSB], [https://www.ebi.ac.uk/pdbsum/3qc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qc9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qc9 RCSB], [http://www.ebi.ac.uk/pdbsum/3qc9 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/GRK1_BOVIN GRK1_BOVIN] Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:12686556, PubMed:16675451, PubMed:21299498). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).[UniProtKB:Q9WVL4]<ref>PMID:12686556</ref> <ref>PMID:16675451</ref> <ref>PMID:21299498</ref>
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G protein-coupled receptor kinases (GRKs) phosphorylate activated G protein-coupled receptors (GPCRs) to initiate receptor desensitization. In addition to the canonical phosphoacceptor site of the kinase domain, activated receptors bind to a distinct docking site that confers higher affinity and activates GRKs allosterically. Recent mutagenesis and structural studies support a model wherein receptor docking activates a GRK by stabilizing the interaction of its ~20 amino acid N-terminal region with the kinase domain. This interaction in turn stabilizes a closed, more active conformation of the enzyme. To investigate the importance of this interaction for the process of GRK activation, we first validated the functionality of the N-terminal region in rhodopsin kinase (GRK1) by site-directed mutagenesis and then introduced a disulfide bond to cross-link the N-terminal region of GRK1 with its specific binding site on the kinase domain. Characterization of the kinetic and biophysical properties of the cross-linked protein showed that disulfide bond formation greatly enhances the catalytic efficiency of the peptide phosphorylation, but receptor-dependent phosphorylation, Meta II stabilization, and inhibition of transducin activation were unaffected. These data indicate that the interaction of the N-terminal region with the kinase domain is important for GRK activation, but that it does not dictate the affinity of GRKs for activated receptors.
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Activation of G protein-coupled receptor kinase 1 involves interactions between its N-terminal region and its kinase domain.,Huang CC, Orban T, Jastrzebska B, Palczewski K, Tesmer JJ Biochemistry. 2011 Jan 25. PMID:21265573<ref>PMID:21265573</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Rhodopsin|Rhodopsin]]
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*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
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*[[Rhodopsin kinase|Rhodopsin kinase]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Rhodopsin kinase]]
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[[Category: Large Structures]]
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[[Category: Huang, C C]]
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[[Category: Huang C-C]]
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[[Category: Tesmer, J J.G]]
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[[Category: Tesmer JJG]]
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[[Category: Enkaryotic protein kinase fold]]
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[[Category: Protein serine/threonine kinase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg

PDB ID 3qc9

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