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3qya

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==Crystal structure of a red-emitter mutant of Lampyris turkestanicus luciferase==
==Crystal structure of a red-emitter mutant of Lampyris turkestanicus luciferase==
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<StructureSection load='3qya' size='340' side='right' caption='[[3qya]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
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<StructureSection load='3qya' size='340' side='right'caption='[[3qya]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qya]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Iranian_firefly Iranian firefly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QYA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qya]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lampyris_turkestanicus Lampyris turkestanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QYA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m46|4m46]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qya OCA], [https://pdbe.org/3qya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qya RCSB], [https://www.ebi.ac.uk/pdbsum/3qya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qya ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qya OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qya RCSB], [http://www.ebi.ac.uk/pdbsum/3qya PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5UFR2_9COLE Q5UFR2_9COLE]
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Firefly bioluminescence reaction in the presence of Mg2+, ATP and molecular oxygen is carried out by luciferase. The luciferase structure alterations or modifications of assay conditions determine the bioluminescence color of firefly luciferase. Among different beetle luciferases, Phrixothrix hirtus railroad worm emits either yellow or red bioluminescence color. Sequence alignment analysis shows that the red-emitter luciferase from P. hirtus has an additional arginine residue at 353 that is absent in other firefly luciferases. It was reported that insertion of Arg in an important flexible loop350-359 showed changes in bioluminescence color from green to red and the optimum temperature activity was also increased. To explain the color tuning mechanism of firefly luciferase, the structure of native and a mutant (E354R/356R/H431Y) of Lampyris turkestanicus luciferase is determined at 2.7A and 2.2A resolutions, respectively. The comparison of structure of both types of L. turkestanicus luciferases reveals that the conformation of this flexible loop is significantly changed by addition of two Arg in this region. Moreover, its surface accessibility is affected considerably and some ionic bonds are made by addition of two positive charge residues. Furthermore, we noticed that the hydrogen bonding pattern of His431 with the flexible loop is changed by replacing this residue with Tyr at this position. Juxtaposition of a flexible loop (residues 351-359) in firefly luciferase and corresponding ionic and hydrogen bonds is essential for color emission.
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Crystal structure of native and a mutant of Lampyris turkestanicus luciferase implicate in bioluminescence color shift.,Kheirabadi M, Sharafian Z, Naderi-Manesh H, Heineman U, Gohlke U, Hosseinkhani S Biochim Biophys Acta. 2013 Oct 6. pii: S1570-9639(13)00356-7. doi:, 10.1016/j.bbapap.2013.09.022. PMID:24103420<ref>PMID:24103420</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Luciferase|Luciferase]]
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*[[Luciferase 3D structures|Luciferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Iranian firefly]]
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[[Category: Lampyris turkestanicus]]
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[[Category: Gohlke, U]]
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[[Category: Large Structures]]
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[[Category: Heinemann, U]]
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[[Category: Gohlke U]]
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[[Category: Khani, S Hossein]]
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[[Category: Heinemann U]]
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[[Category: Kheirabadi, M]]
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[[Category: Hossein Khani S]]
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[[Category: Naderi-Manesh, H]]
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[[Category: Kheirabadi M]]
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[[Category: Acyl-coenzyme a ligase]]
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[[Category: Naderi-Manesh H]]
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[[Category: Atp binding]]
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[[Category: Bioluminescence]]
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[[Category: Light emitting]]
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[[Category: Luciferin-binding]]
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[[Category: Mutagenesis]]
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[[Category: Oxidoreductase]]
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[[Category: Peroxisome]]
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Current revision

Crystal structure of a red-emitter mutant of Lampyris turkestanicus luciferase

PDB ID 3qya

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