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3r3m

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==Crystal structure of the FAF1 UBX domain==
==Crystal structure of the FAF1 UBX domain==
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<StructureSection load='3r3m' size='340' side='right' caption='[[3r3m]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='3r3m' size='340' side='right'caption='[[3r3m]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3r3m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R3M FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3r3m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R3M FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FAF1, UBXD12, UBXN3A, CGI-03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3m OCA], [http://pdbe.org/3r3m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r3m RCSB], [http://www.ebi.ac.uk/pdbsum/3r3m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r3m ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3m OCA], [https://pdbe.org/3r3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r3m RCSB], [https://www.ebi.ac.uk/pdbsum/3r3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r3m ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/FAF1_HUMAN FAF1_HUMAN]] Potentiates but cannot initiate FAS-induced apoptosis.
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[https://www.uniprot.org/uniprot/FAF1_HUMAN FAF1_HUMAN] Potentiates but cannot initiate FAS-induced apoptosis.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hexameric AAA ATPase p97 is involved in several human proteinopathies and mediates ubiquitin-dependent protein degradation among other essential cellular processes. Via its N-terminal domain (N domain), p97 interacts with multiple regulatory cofactors including the UFD1/NPL4 heterodimer and members of the "ubiquitin regulatory X" (UBX) domain protein family; however, the principles governing cofactor selectivity remain to be deciphered. Our crystal structure of the FAS-associated factor 1 (FAF1)UBX domain in complex with the p97N domain reveals that the signature Phe-Pro-Arg motif known to be crucial for interactions of UBX domains with p97 adopts a cis-proline configuration, in contrast to a cis-trans mixture we derive for the isolated FAF1UBX domain. Biochemical studies confirm that binding critically depends on a proline at this position. Furthermore, we observe that the UBX proteins FAF1 and UBXD7 only bind to p97-UFD1/NPL4, but not free p97, thus demonstrating for the first time a hierarchy in p97-cofactor interactions.
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Hierarchical Binding of Cofactors to the AAA ATPase p97.,Hanzelmann P, Buchberger A, Schindelin H Structure. 2011 Jun 8;19(6):833-43. PMID:21645854<ref>PMID:21645854</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3r3m" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
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[[Category: Haenzelmann, P]]
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[[Category: Large Structures]]
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[[Category: Schindelin, H]]
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[[Category: Haenzelmann P]]
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[[Category: Apoptosis]]
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[[Category: Schindelin H]]
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[[Category: Beta grasp fold]]
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[[Category: P97]]
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[[Category: Phosphorylation]]
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Current revision

Crystal structure of the FAF1 UBX domain

PDB ID 3r3m

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