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| | ==Rad60 SLD2 Ubc9 Complex== | | ==Rad60 SLD2 Ubc9 Complex== |
| - | <StructureSection load='3rcz' size='340' side='right' caption='[[3rcz]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3rcz' size='340' side='right'caption='[[3rcz]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rcz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rcz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RCZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3goe|3goe]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rad60, SPBC1921.02 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), hus5, ubc9, SPAC30D11.13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcz OCA], [https://pdbe.org/3rcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rcz RCSB], [https://www.ebi.ac.uk/pdbsum/3rcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcz RCSB], [http://www.ebi.ac.uk/pdbsum/3rcz PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RAD60_SCHPO RAD60_SCHPO]] Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.<ref>PMID:11971984</ref> <ref>PMID:12897162</ref> [[http://www.uniprot.org/uniprot/UBC9_SCHPO UBC9_SCHPO]] Catalyzes the covalent attachment of ubiquitin-like protein SUMO/Smt3 to other proteins. Required for efficient recovery from DNA damage or S-phase arrest and normal mitosis. This may be as part of a checkpoint independent recovery process.<ref>PMID:12597774</ref> <ref>PMID:7768995</ref> | + | [https://www.uniprot.org/uniprot/RAD60_SCHPO RAD60_SCHPO] Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.<ref>PMID:11971984</ref> <ref>PMID:12897162</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Global sumoylation, SUMO chain formation and genome stabilization are all outputs generated by a limited repertoire of enzymes. Mechanisms driving selectivity for each of these processes are largely uncharacterized. Here, through crystallographic analyses we show that the SUMO E2 Ubc9 forms a non-covalent complex with a SUMO-like domain of Rad60 (SLD2). Ubc9:SLD2 and Ubc9:SUMO non-covalent complexes are structurally analogous, suggesting that differential recruitment of Ubc9 by SUMO or Rad60 provides a novel means for such selectivity. Indeed, deconvoluting Ubc9 function by disrupting either the Ubc9:SLD2 or Ubc9:SUMO non-covalent complex reveals distinct roles in facilitating sumoylation. Ubc9:SLD2 acts in the Nse2 SUMO E3 ligase-dependent pathway for DNA repair, whereas Ubc9:SUMO instead promotes global sumoylation and chain formation, via the Pli1 E3 SUMO ligase. Moreover, this Pli1-dependent SUMO chain formation causes the genome instability phenotypes of SUMO-targeted ubiquitin ligase (STUbL) mutants. Overall, we determine that unexpectedly, Ubc9 non-covalent partner choice dictates the role of sumoylation in distinct cellular pathways.
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| - | DNA Repair and Global Sumoylation are Regulated by Distinct Ubc9 Non-covalent Complexes.,Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN Mol Cell Biol. 2011 Mar 28. PMID:21444718<ref>PMID:21444718</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| | ==See Also== | | ==See Also== |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Schizosaccharomyces pombe]] | | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: Arvai, A S]] | + | [[Category: Arvai AS]] |
| - | [[Category: Perry, J J.P]] | + | [[Category: Perry JJP]] |
| - | [[Category: Tainer, J A]] | + | [[Category: Tainer JA]] |
| - | [[Category: Protein binding-ligase complex]]
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| - | [[Category: Protein:protein interaction]]
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| - | [[Category: Sumo-like domain]]
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| - | [[Category: Ubc9]]
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