This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3re8

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Structural and Kinetic Analysis of the Beef liver Catalase interacting with Nitric Oxide

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3re8"

Categories: Bos taurus | Large Structures | Purwar N | Schmidt M

@@ Line 1: / Line 1: @@
 ==Structural and Kinetic Analysis of the Beef liver Catalase interacting with Nitric Oxide==
-<StructureSection load='3re8' size='340' side='right' caption='[[3re8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='3re8' size='340' side='right'caption='[[3re8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3re8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RE8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3re8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RE8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rgp|3rgp]], [[3rgs|3rgs]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3re8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3re8 OCA], [https://pdbe.org/3re8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3re8 RCSB], [https://www.ebi.ac.uk/pdbsum/3re8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3re8 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3re8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3re8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3re8 RCSB], [http://www.ebi.ac.uk/pdbsum/3re8 PDBsum]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/CATA_BOVIN CATA_BOVIN] Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
-We present the structures of bovine catalase in its native form and complexed with ammonia and nitric oxide, obtained by X-ray crystallography. Using the NO generator 1-(N,N-diethylamino)diazen-1-ium-1,2-diolate, we were able to generate sufficiently high NO concentrations within the catalase crystals that substantial occupation was observed despite a high dissociation rate. Nitric oxide seems to be slightly bent from the heme normal that may indicate some iron(II) character in the formally ferric catalase. Microspectrophotometric investigations inline with the synchrotron X-ray beam reveal photoreduction of the central heme iron. In the cases of the native and ammonia-complexed catalase, reduction is accompanied by a relaxation phase. This is likely not the case for the catalase NO complex. The kinetics of binding of NO to catalase were investigated using NO photolyzed from N,N'-bis(carboxymethyl)-N,N'-dinitroso-p-phenylenediamine using an assay that combines catalase with myoglobin binding kinetics. The off rate is 1.5 s(-1). Implications for catalase function are discussed.
-Interaction of Nitric Oxide with Catalase: Structural and Kinetic Analysis.,Purwar N, McGarry JM, Kostera J, Pacheco AA, Schmidt M Biochemistry. 2011 May 6. PMID:21524057<ref>PMID:21524057</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
 ==See Also==
-*[[Catalase|Catalase]]
+*[[Catalase 3D structures|Catalase 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Bos taurus]]
-[[Category: Catalase]]
+[[Category: Large Structures]]
-[[Category: Purwar, N]]
+[[Category: Purwar N]]
-[[Category: Schmidt, M]]
+[[Category: Schmidt M]]
-[[Category: Disproportionation of the hydrogen peroxide]]
-[[Category: Oxidoreducatase]]
-[[Category: Oxidoreductase]]
-[[Category: The 5-coordinated heme protein]]

3re8

From Proteopedia

Current revision

Structural and Kinetic Analysis of the Beef liver Catalase interacting with Nitric Oxide

Structural highlights

Function

See Also

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox