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3ru8

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==Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab==
==Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab==
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<StructureSection load='3ru8' size='340' side='right' caption='[[3ru8]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
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<StructureSection load='3ru8' size='340' side='right'caption='[[3ru8]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ru8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aids_virus Aids virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RU8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ru8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus], [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RU8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ru8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ru8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ru8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ru8 PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ru8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ru8 OCA], [https://pdbe.org/3ru8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ru8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ru8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ru8 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Disease ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/IGKC_HUMAN IGKC_HUMAN] Defects in IGKC are the cause of immunoglobulin kappa light chain deficiency (IGKCD) [MIM:[https://omim.org/entry/614102 614102]. IGKCD is a disease characterized by the complete absence of immunoglobulin kappa chains.<ref>PMID:3931219</ref>
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The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_HUMAN IGKC_HUMAN]
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Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.,Azoitei ML, Correia BE, Ban YE, Carrico C, Kalyuzhniy O, Chen L, Schroeter A, Huang PS, McLellan JS, Kwong PD, Baker D, Strong RK, Schief WR Science. 2011 Oct 21;334(6054):373-6. PMID:22021856<ref>PMID:22021856</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Antibody|Antibody]]
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[3D structures of human antibody|3D structures of human antibody]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aids virus]]
 
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Carrico, C T.D]]
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[[Category: Human immunodeficiency virus]]
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[[Category: Strong, R K]]
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[[Category: Large Structures]]
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[[Category: Binding epitope from hiv on a heterologous protein]]
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[[Category: Synthetic construct]]
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[[Category: De novo protein]]
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[[Category: Thermobifida fusca]]
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[[Category: Immune system]]
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[[Category: Carrico CTD]]
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[[Category: Strong RK]]

Current revision

Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab

PDB ID 3ru8

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