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3sj0

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==PpcA mutant M58S==
==PpcA mutant M58S==
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<StructureSection load='3sj0' size='340' side='right' caption='[[3sj0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='3sj0' size='340' side='right'caption='[[3sj0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3sj0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SJ0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3sj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SJ0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sel|3sel]], [[3sj1|3sj1]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ppcA, GSU0612 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35554 Geobacter sulfurreducens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj0 OCA], [https://pdbe.org/3sj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sj0 RCSB], [https://www.ebi.ac.uk/pdbsum/3sj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj0 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sj0 RCSB], [http://www.ebi.ac.uk/pdbsum/3sj0 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8GGK7_GEOSN Q8GGK7_GEOSN]
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PpcA is a small protein with 71 residues that contains three covalently bound hemes. The structures of single mutants at residue 58 have shown larger deviations in another part of the protein molecule than at the site of the mutation. Closer examination of the crystal packing has revealed the origin of this unexpected structural change. The site of mutation is within Van der Waals distance from another protein molecule related by a crystallographic twofold axis within the crystal. The structural changes occurred at or near the mutation site have led to a slight adjustment of the surface residues in contact. The observed deviations between the native and the mutant molecular structures are derived from the new crystal packing even though the two crystals are essentially isomorphous. Without careful consideration of the crystal lattice a non-expert looking at only the coordinates deposited in the Protein Data Bank could draw erroneous conclusion that mutation in one part of the molecule affected the structure of the protein in a distant part of the molecule.
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Pitfalls in the interpretation of structural changes in mutant proteins from crystal structures.,Pokkuluri PR, Yang X, Londer YY, Schiffer M J Struct Funct Genomics. 2012 Dec;13(4):227-32. doi: 10.1007/s10969-012-9147-1., Epub 2012 Oct 26. PMID:23099666<ref>PMID:23099666</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Geobacter sulfurreducens]]
[[Category: Geobacter sulfurreducens]]
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[[Category: Pokkuluri, P R]]
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[[Category: Large Structures]]
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[[Category: Schiffer, M]]
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[[Category: Pokkuluri PR]]
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[[Category: Electron transport]]
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[[Category: Schiffer M]]
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[[Category: Three heme cytochrome c7]]
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Current revision

PpcA mutant M58S

PDB ID 3sj0

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