|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of an H-NOX protein from T. tengcongensis== | | ==Crystal structure of an H-NOX protein from T. tengcongensis== |
| - | <StructureSection load='3tf0' size='340' side='right' caption='[[3tf0]], [[Resolution|resolution]] 1.74Å' scene=''> | + | <StructureSection load='3tf0' size='340' side='right'caption='[[3tf0]], [[Resolution|resolution]] 1.74Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tf0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_1.2430 As 1.2430]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TF0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tf0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TF0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.743Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u55|1u55]], [[1u56|1u56]], [[1u4h|1u4h]], [[3tf1|3tf1]], [[3tf8|3tf8]], [[3tf9|3tf9]], [[3tfa|3tfa]], [[3tfd|3tfd]], [[3tfe|3tfe]], [[3tff|3tff]], [[3tfg|3tfg]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tar4, TTE0680 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119072 AS 1.2430])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tf0 OCA], [https://pdbe.org/3tf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tf0 RCSB], [https://www.ebi.ac.uk/pdbsum/3tf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tf0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tf0 OCA], [http://pdbe.org/3tf0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tf0 RCSB], [http://www.ebi.ac.uk/pdbsum/3tf0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tf0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4] |
| - | Interior topological features, such as pockets and channels, have evolved in proteins to regulate biological functions by facilitating the diffusion of biomolecules. Decades of research using the globins as model heme proteins have clearly highlighted the importance of gas pockets around the heme in controlling the capture and release of O(2). However, much less is known about how ligand migration contributes to the diverse functions of other heme protein scaffolds. Heme nitric oxide/oxygen binding (H-NOX) domains are a conserved family of gas-sensing heme proteins with a divergent fold that are critical to numerous signaling pathways. Utilizing X-ray crystallography with xenon, a tunnel network has been shown to serve as a molecular pathway for ligand diffusion. Structure-guided mutagenesis results show that the tunnels have unexpected effects on gas-sensing properties in H-NOX domains. The findings provide insights on how the flux of biomolecules through protein scaffolds modulates protein chemistry.
| + | |
| | | | |
| - | Tunnels modulate ligand flux in a heme nitric oxide/oxygen binding (H-NOX) domain.,Winter MB, Herzik MA Jr, Kuriyan J, Marletta MA Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):E881-9. Epub 2011 Oct 12. PMID:21997213<ref>PMID:21997213</ref>
| + | ==See Also== |
| - | | + | *[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3tf0" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: As 1 2430]] | + | [[Category: Caldanaerobacter subterraneus subsp. tengcongensis]] |
| - | [[Category: Herzik, M A]] | + | [[Category: Large Structures]] |
| - | [[Category: Kuriyan, J]] | + | [[Category: Herzik Jr MA]] |
| - | [[Category: Marletta, M A]] | + | [[Category: Kuriyan J]] |
| - | [[Category: Winter, M B]] | + | [[Category: Marletta MA]] |
| - | [[Category: Gas binding]] | + | [[Category: Winter MB]] |
| - | [[Category: Heme-based methyl-accepting chemotaxis protein]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
