This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3tlc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:32, 14 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 3: Line 3:
<StructureSection load='3tlc' size='340' side='right'caption='[[3tlc]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
<StructureSection load='3tlc' size='340' side='right'caption='[[3tlc]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3tlc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLC FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3tlc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLC FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7MD:5-O-[(R)-(3-AMINOPROPOXY)(L-ALPHA-ASPARTYLAMINO)PHOSPHORYL]ADENOSINE'>7MD</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tla|3tla]], [[3tlb|3tlb]], [[3tle|3tle]], [[3tlg|3tlg]], [[3tly|3tly]], [[3tlz|3tlz]]</div></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7MD:5-O-[(R)-(3-AMINOPROPOXY)(L-ALPHA-ASPARTYLAMINO)PHOSPHORYL]ADENOSINE'>7MD</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mccF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlc OCA], [https://pdbe.org/3tlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tlc RCSB], [https://www.ebi.ac.uk/pdbsum/3tlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tlc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlc OCA], [https://pdbe.org/3tlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tlc RCSB], [https://www.ebi.ac.uk/pdbsum/3tlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tlc ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/MCCF_ECOLX MCCF_ECOLX] Involved in specific self-immunity to microcin C7.
-
Several classes of naturally occurring antimicrobials exert their antibiotic activity by specifically targeting aminoacyl-tRNA synthetases, validating these enzymes as drug targets. The aspartyl tRNA synthetase "Trojan horse" inhibitor microcin C7 (McC7) consists of a nonhydrolyzable aspartyl-adenylate conjugated to a hexapeptide carrier that facilitates active import into bacterial cells through an oligopeptide transport system. Subsequent proteolytic processing releases the toxic compound inside the cell. Producing strains of McC7 must protect themselves against autotoxicity that may result from premature processing. The mccF gene confers resistance against endogenous and exogenous McC7 by hydrolyzing the amide bond that connects the peptide and nucleotide moieties of McC7. We present here crystal structures of MccF, in complex with various ligands. The MccF structure is similar to that of dipeptide ld-carboxypeptidase, but with an additional loop proximal to the active site that serves as the primary determinant for recognition of adenylated substrates. Wild-type MccF only hydrolyzes the naturally occurring aspartyl phosphoramidate McC7 and synthetic peptidyl sulfamoyl adenylates that contain anionic side chains. We show that substitutions of two active site MccF residues result in a specificity switch toward aromatic aminoacyl-adenylate substrates. These results suggest how MccF-like enzymes may be used to avert various toxic aminoacyl-adenylates that accumulate during antibiotic biosynthesis or in normal metabolism of the cell.
+
-
 
+
-
Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3tlc" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacillus coli migula 1895]]
+
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Agarwal, V]]
+
[[Category: Agarwal V]]
-
[[Category: Nair, S K]]
+
[[Category: Nair SK]]
-
[[Category: Hydrolase-antibiotic complex]]
+
-
[[Category: Serine protease]]
+

Current revision

Microcin C7 self immunity protein MccF in complex with Microcin C7 antibiotic

PDB ID 3tlc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tlc"
Personal tools