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| | ==Crystal structure of TrmN from Thermus thermophilus== | | ==Crystal structure of TrmN from Thermus thermophilus== |
| - | <StructureSection load='3tma' size='340' side='right' caption='[[3tma]], [[Resolution|resolution]] 2.05Å' scene=''> | + | <StructureSection load='3tma' size='340' side='right'caption='[[3tma]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tma]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TMA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TMA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tma]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TMA FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tlj|3tlj]], [[3tm4|3tm4]], [[3tm5|3tm5]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTC1157, TT_C1157 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tma OCA], [https://pdbe.org/3tma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tma RCSB], [https://www.ebi.ac.uk/pdbsum/3tma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tma ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tma OCA], [http://pdbe.org/3tma PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tma RCSB], [http://www.ebi.ac.uk/pdbsum/3tma PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tma ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/TRMN_THET2 TRMN_THET2] S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Phe).<ref>PMID:22337946</ref> <ref>PMID:22362751</ref> |
| - | Methyltransferases (MTases) form a major class of tRNA-modifying enzymes needed for the proper functioning of tRNA. Recently, RNA MTases from the TrmN/Trm14 family that are present in Archaea, Bacteria and Eukaryota have been shown to specifically modify tRNA(Phe) at guanosine 6 in the tRNA acceptor stem. Here, we report the first X-ray crystal structures of the tRNA m(2)G6 (N(2)-methylguanosine) MTase (TTC)TrmN from Thermus thermophilus and its ortholog (Pf)Trm14 from Pyrococcus furiosus. Structures of (Pf)Trm14 were solved in complex with the methyl donor S-adenosyl-l-methionine (SAM or AdoMet), as well as the reaction product S-adenosyl-homocysteine (SAH or AdoHcy) and the inhibitor sinefungin. (TTC)TrmN and (Pf)Trm14 consist of an N-terminal THUMP domain fused to a catalytic Rossmann-fold MTase (RFM) domain. These results represent the first crystallographic structure analysis of proteins containing both THUMP and RFM domain, and hence provide further insight in the contribution of the THUMP domain in tRNA recognition and catalysis. Electrostatics and conservation calculations suggest a main tRNA binding surface in a groove between the THUMP domain and the MTase domain. This is further supported by a docking model of TrmN in complex with tRNA(Phe) of T. thermophilus and via site-directed mutagenesis.
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| - | Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.,Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versees W Nucleic Acids Res. 2012 Feb 22. PMID:22362751<ref>PMID:22362751</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3tma" style="background-color:#fffaf0;"></div>
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| - | | + | |
| - | ==See Also==
| + | |
| - | *[[TRNA:m2G6 methyltransferase TrmN/Trm14|TRNA:m2G6 methyltransferase TrmN/Trm14]]
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thet2]] | + | [[Category: Large Structures]] |
| - | [[Category: Bujnicki, J M]] | + | [[Category: Thermus thermophilus HB27]] |
| - | [[Category: Droogmans, L]] | + | [[Category: Bujnicki JM]] |
| - | [[Category: Fislage, M]] | + | [[Category: Droogmans L]] |
| - | [[Category: Roovers, M]] | + | [[Category: Fislage M]] |
| - | [[Category: Tuszynska, I]] | + | [[Category: Roovers M]] |
| - | [[Category: Versees, W]] | + | [[Category: Tuszynska I]] |
| - | [[Category: Rossmann fold methyltransferase]]
| + | [[Category: Versees W]] |
| - | [[Category: Thump domain]]
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| - | [[Category: Transferase]]
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| - | [[Category: Trna methyltransferase]]
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