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3u40

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==Crystal structure of a purine nucleoside phosphorylase from Entamoeba histolytica bound to adenosine==
==Crystal structure of a purine nucleoside phosphorylase from Entamoeba histolytica bound to adenosine==
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<StructureSection load='3u40' size='340' side='right' caption='[[3u40]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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<StructureSection load='3u40' size='340' side='right'caption='[[3u40]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3u40]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U40 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3u40]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U40 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tl6|3tl6]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EHI_130960 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u40 OCA], [https://pdbe.org/3u40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u40 RCSB], [https://www.ebi.ac.uk/pdbsum/3u40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u40 ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u40 OCA], [http://pdbe.org/3u40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u40 RCSB], [http://www.ebi.ac.uk/pdbsum/3u40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u40 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/C4LXG4_ENTH1 C4LXG4_ENTH1]
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Despite recent advances, the expression of heterologous proteins in Escherichia coli for crystallization remains a nontrivial challenge. The present study investigates the efficacy of maltose-binding protein (MBP) fusion as a general strategy for rescuing the expression of target proteins. From a group of sequence-verified clones with undetectable levels of protein expression in an E. coli T7 expression system, 95 clones representing 16 phylogenetically diverse organisms were selected for recloning into a chimeric expression vector with an N-terminal histidine-tagged MBP. PCR-amplified inserts were annealed into an identical ligation-independent cloning region in an MBP-fusion vector and were analyzed for expression and solubility by high-throughput nickel-affinity binding. This approach yielded detectable expression of 72% of the clones; soluble expression was visible in 62%. However, the solubility of most proteins was marginal to poor upon cleavage of the MBP tag. This study offers large-scale evidence that MBP can improve the soluble expression of previously non-expressing proteins from a variety of eukaryotic and prokaryotic organisms. While the behavior of the cleaved proteins was disappointing, further refinements in MBP tagging may permit the more widespread use of MBP-fusion proteins in crystallographic studies.
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Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.,Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1006-9. Epub 2011 Aug 13. PMID:21904041<ref>PMID:21904041</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3u40" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Purine nucleoside phosphorylase|Purine nucleoside phosphorylase]]
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*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Enthi]]
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[[Category: Entamoeba histolytica]]
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[[Category: Purine-nucleoside phosphorylase]]
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[[Category: Large Structures]]
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[[Category: Edwards, T E]]
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[[Category: Edwards TE]]
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[[Category: Gardberg, A S]]
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[[Category: Gardberg AS]]
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[[Category: Structural genomic]]
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[[Category: Co-crystal]]
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[[Category: Expression rescue]]
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[[Category: Maltose binding protein]]
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[[Category: Purine salvage]]
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[[Category: Ssgcid]]
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[[Category: Transferase]]
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Current revision

Crystal structure of a purine nucleoside phosphorylase from Entamoeba histolytica bound to adenosine

PDB ID 3u40

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