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3uq4

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==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant F247L (F16L)==
==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant F247L (F16L)==
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<StructureSection load='3uq4' size='340' side='right' caption='[[3uq4]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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<StructureSection load='3uq4' size='340' side='right'caption='[[3uq4]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3uq4]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3uq4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ4 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3uq5|3uq5]], [[3uq7|3uq7]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq4 OCA], [https://pdbe.org/3uq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq4 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq4 ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uq4 RCSB], [http://www.ebi.ac.uk/pdbsum/3uq4 PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3]
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== Publication Abstract from PubMed ==
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The determination of structural models of the various stable states of an ion channel is a key step toward the characterization of its conformational dynamics. In the case of nicotinic-type receptors, different structures have been solved but, thus far, these different models have been obtained from different members of the superfamily. In the case of the bacterial member ELIC, a cysteamine-gated channel from Erwinia chrisanthemi, a structural model of the protein in the absence of activating ligand (and thus, conceivably corresponding to the closed state of this channel) has been previously generated. In this article, electrophysiological characterization of ELIC mutants allowed us to identify pore mutations that slow down the time course of desensitization to the extent that the channel seems not to desensitize at all for the duration of the agonist applications (&gt;20 min). Thus, it seems reasonable to conclude that the probability of ELIC occupying the closed state is much lower for the ligand-bound mutants than for the unliganded wild-type channel. To gain insight into the conformation adopted by ELIC under these conditions, we solved the crystal structures of two of these mutants in the presence of a concentration of cysteamine that elicits an intracluster open probability of &gt;0.9. Curiously, the obtained structural models turned out to be nearly indistinguishable from the model of the wild-type channel in the absence of bound agonist. Overall, our findings bring to light the limited power of functional studies in intact membranes when it comes to inferring the functional state of a channel in a crystal, at least in the case of the nicotinic-receptor superfamily.
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Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.,Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C Proc Natl Acad Sci U S A. 2012 Apr 2. PMID:22474383<ref>PMID:22474383</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Erwinia chrysanthemi]]
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[[Category: Dickeya dadantii 3937]]
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[[Category: Agarwal, V.]]
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[[Category: Large Structures]]
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[[Category: Gonzalez-Gutierrez, G.]]
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[[Category: Agarwal V]]
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[[Category: Grosman, C.]]
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[[Category: Gonzalez-Gutierrez G]]
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[[Category: Lukk, T.]]
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[[Category: Grosman C]]
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[[Category: Nair, S K.]]
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[[Category: Lukk T]]
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[[Category: Papke, D.]]
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[[Category: Nair SK]]
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[[Category: Liganded-gated ion channel]]
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[[Category: Papke D]]
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[[Category: Membrane protein]]
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[[Category: Transport protein]]
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Current revision

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant F247L (F16L)

PDB ID 3uq4

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