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3uzz

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[[Image:3uzz.png|left|200px]]
 
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{{STRUCTURE_3uzz| PDB=3uzz | SCENE= }}
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==Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and delta4-androstenedione==
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<StructureSection load='3uzz' size='340' side='right'caption='[[3uzz]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3uzz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UZZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASD:4-ANDROSTENE-3-17-DIONE'>ASD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=TES:TESTOSTERONE'>TES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzz OCA], [https://pdbe.org/3uzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uzz RCSB], [https://www.ebi.ac.uk/pdbsum/3uzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uzz ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/AK1D1_HUMAN AK1D1_HUMAN] Defects in AKR1D1 are the cause of congenital bile acid synthesis defect type 2 (CBAS2) [MIM:[https://omim.org/entry/235555 235555]; also known as cholestasis with delta(4)-3-oxosteroid 5-beta-reductase deficiency. Patients with this liver disease show absence or low levels of chenodeoxycholic acid and cholic acid in plasma and urine.<ref>PMID:12970144</ref> <ref>PMID:15030995</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/AK1D1_HUMAN AK1D1_HUMAN] Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
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===Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and delta4-androstenedione===
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==See Also==
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*[[Aldo-keto reductase 3D structures|Aldo-keto reductase 3D structures]]
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{{ABSTRACT_PUBMED_22437839}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3uzz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZZ OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:022437839</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Chen, M.]]
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[[Category: Large Structures]]
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[[Category: Christianson, D W.]]
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[[Category: Chen M]]
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[[Category: Penning, T M.]]
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[[Category: Christianson DW]]
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[[Category: Aldo-keto reductase]]
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[[Category: Penning TM]]
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[[Category: Catalytic tetrad mutant]]
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[[Category: Oxidoreductase]]
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[[Category: Steroid and bile acid metabolism]]
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[[Category: Tim barrel]]
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Current revision

Crystal structure of 5beta-reductase (AKR1D1) E120H mutant in complex with NADP+ and delta4-androstenedione

PDB ID 3uzz

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