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3v7a

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==Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle==
==Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle==
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<StructureSection load='3v7a' size='340' side='right' caption='[[3v7a]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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<StructureSection load='3v7a' size='340' side='right'caption='[[3v7a]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3v7a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_calicivirus_nlv Human calicivirus nlv] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V7A FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3v7a]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Norovirus_isolates Norovirus isolates]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V7A FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3onu|3onu]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.297&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7a OCA], [http://pdbe.org/3v7a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v7a RCSB], [http://www.ebi.ac.uk/pdbsum/3v7a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v7a ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7a OCA], [https://pdbe.org/3v7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v7a RCSB], [https://www.ebi.ac.uk/pdbsum/3v7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v7a ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5F4T5_9CALI Q5F4T5_9CALI]
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Human noroviruses are genetically and antigenically highly divergent. Monoclonal antibodies raised in mice against one kind of norovirus virus-like particle (VLP), however, were found to have broad recognition. In this study, we present the crystal structure of the antigen-binding fragment (Fab) for one of these broadly reactive monoclonal antibodies, 5B18, in complex with the capsid-protruding domain from a genogroup II genotype 10 (GII.10) norovirus at 3.3 A resolution and also the cryo-electron microscopy structure of the GII.10 VLP at approximately 10 A resolution. The GII.10 VLP structure was more similar in overall architecture to the GV.1 murine norovirus virion than to the prototype GI.1 human norovirus VLP, with the GII.10 protruding domain raised approximately 15 A off the shell domain and rotated approximately 40 degrees relative to the GI.1 protruding domain. In the crystal structure, the 5B18 Fab bound to a highly conserved region of the protruding domain. Based on the VLP structure, this region is involved in interactions with other regions of the capsid and is partially buried in the virus particle. Despite the occluded nature of the recognized epitope in the VLP structure, ELISA binding suggested that the 5B18 antibody was able to capture intact VLPs. Together, the results provide evidence that the norovirus particle is capable of conformational flexibility, which may allow for antibody recognition of conserved surfaces that would otherwise be deeply buried on intact norovirus particles.
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Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle.,Hansman GS, Taylor DW, McLellan JS, Smith TJ, Georgiev I, Tame JR, Park SY, Yamazaki M, Gondaira F, Miki M, Katayama K, Murata K, Kwong PD J Virol. 2012 Jan 25. PMID:22278249<ref>PMID:22278249</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3v7a" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Virus coat protein|Virus coat protein]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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*[[3D structures of monoclonal antibody|3D structures of monoclonal antibody]]
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human calicivirus nlv]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Hansman, G S]]
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[[Category: Norovirus isolates]]
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[[Category: Kwong, P D]]
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[[Category: Hansman GS]]
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[[Category: Mclellan, J S]]
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[[Category: Kwong PD]]
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[[Category: Broadly-reactive antibody]]
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[[Category: Mclellan JS]]
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[[Category: Immune system]]
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[[Category: Protein-fab complex]]
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[[Category: Virus]]
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Current revision

Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle

PDB ID 3v7a

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