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4dam

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==Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor==
==Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor==
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<StructureSection load='4dam' size='340' side='right' caption='[[4dam]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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<StructureSection load='4dam' size='340' side='right'caption='[[4dam]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4dam]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DAM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4dam]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DAM FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3eiv|3eiv]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCC61A.04c, SCO2683, ssb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dam OCA], [https://pdbe.org/4dam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dam RCSB], [https://www.ebi.ac.uk/pdbsum/4dam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dam ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dam OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dam RCSB], [http://www.ebi.ac.uk/pdbsum/4dam PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/SSB1_STRCO SSB1_STRCO] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity).
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The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Following mutational analysis, we concluded that ssbA is essential, whereas ssbB plays a key role in chromosome segregation during sporulation. In the ssbB mutant, approximately 30% of spores lacked DNA. The two ssb genes were expressed differently; in minimal medium, gene expression was prolonged for both genes and significantly upregulated for ssbB. The ssbA gene is transcribed as part of a polycistronic mRNA from two initiation sites, 163 bp and 75 bp upstream of the rpsF translational start codon. The ssbB gene is transcribed as a monocistronic mRNA, from an unusual promoter region, 73 bp upstream of the AUG codon. Distinctive DNA-binding affinities of single-stranded DNA-binding proteins monitored by tryptophan fluorescent quenching and electrophoretic mobility shift were observed. The crystal structure of SsbB at 1.7 A resolution revealed a common OB-fold, lack of the clamp-like structure conserved in SsbA and previously unpublished S-S bridges between the A/B and C/D subunits. This is the first report of the determination of paralogous single-stranded DNA-binding protein structures from the same organism. Phylogenetic analysis revealed frequent duplication of ssb genes in Actinobacteria, whereas their strong retention suggests that they are involved in important cellular functions.
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Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.,Paradzik T, Ivic N, Filic Z, Manjasetty BA, Herron P, Luic M, Vujaklija D Nucleic Acids Res. 2013 Apr 1;41(6):3659-72. doi: 10.1093/nar/gkt050. Epub 2013, Feb 7. PMID:23393191<ref>PMID:23393191</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces coelicolor]]
[[Category: Streptomyces coelicolor]]
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[[Category: Filic, Z]]
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[[Category: Filic Z]]
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[[Category: Herron, P]]
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[[Category: Herron P]]
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[[Category: Ivic, N]]
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[[Category: Ivic N]]
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[[Category: Luic, M]]
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[[Category: Luic M]]
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[[Category: Manjasetty, B A]]
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[[Category: Manjasetty BA]]
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[[Category: Paradzik, T]]
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[[Category: Paradzik T]]
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[[Category: Vujaklija, D]]
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[[Category: Vujaklija D]]
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[[Category: Dna binding protein]]
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[[Category: Dna-binding]]
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[[Category: Ob-fold]]
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[[Category: Single-stranded dna]]
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Current revision

Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor

PDB ID 4dam

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