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4doe

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The liganded structure of Cbescii CelA GH9 module

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Retrieved from "http://52.214.119.220/wiki/index.php/4doe"

Categories: Caldicellulosiruptor bescii | Large Structures | Alahuhta PM | Lunin VV

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 == Structural highlights ==
 <table><tr><td colspan='2'>[[4doe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldicellulosiruptor_bescii Caldicellulosiruptor bescii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DOE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900021:beta-cellotriose'>PRD_900021</scene>, <scene name='pdbligand=PRD_900023:alpha-cellobiose'>PRD_900023</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.561&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900021:beta-cellotriose'>PRD_900021</scene>, <scene name='pdbligand=PRD_900023:alpha-cellobiose'>PRD_900023</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4doe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4doe OCA], [https://pdbe.org/4doe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4doe RCSB], [https://www.ebi.ac.uk/pdbsum/4doe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4doe ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/P96311_CALBS P96311_CALBS]]
+[https://www.uniprot.org/uniprot/P96311_CALBS P96311_CALBS]
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Most fungi and bacteria degrade plant cell walls by secreting free, complementary enzymes that hydrolyze cellulose; however, some bacteria use large enzymatic assemblies called cellulosomes, which recruit complementary enzymes to protein scaffolds. The thermophilic bacterium Caldicellulosiruptor bescii uses an intermediate strategy, secreting many free cellulases that contain multiple catalytic domains. One of these, CelA, comprises a glycoside hydrolase family 9 and a family 48 catalytic domain, as well as three type III cellulose-binding modules. In the saccharification of a common cellulose standard, Avicel, CelA outperforms mixtures of commercially relevant exo- and endoglucanases. From transmission electron microscopy studies of cellulose after incubation with CelA, we report morphological features that suggest that CelA not only exploits the common surface ablation mechanism driven by general cellulase processivity, but also excavates extensive cavities into the surface of the substrate. These results suggest that nature's repertoire of cellulose digestion paradigms remain only partially discovered and understood.
-Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA.,Brunecky R, Alahuhta M, Xu Q, Donohoe BS, Crowley MF, Kataeva IA, Yang SJ, Resch MG, Adams MW, Lunin VV, Himmel ME, Bomble YJ Science. 2013 Dec 20;342(6165):1513-6. doi: 10.1126/science.1244273. PMID:24357319<ref>PMID:24357319</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 4doe" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Glucanase 3D structures|Glucanase 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>

4doe

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The liganded structure of Cbescii CelA GH9 module

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