4eiv

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==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
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<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
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<StructureSection load='4eiv' size='340' side='right'caption='[[4eiv]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qyq|3qyq]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [https://pdbe.org/4eiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [https://www.ebi.ac.uk/pdbsum/4eiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiv ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B9PVB4_TOXGV B9PVB4_TOXGV]
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Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals world-wide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii, a highly successful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent bradyzoite stage. While the importance of TgALD1 in glycolysis is well established and TgDERA is also likely to be involved in parasite metabolism, the detailed function of TgDPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of TgALD1 and TgDPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of TgALD1 and TgDPA with members of their respective enzyme families revealed that while the active site residues are conserved in TgALD1, key catalytic residues are absent in TgDPA. Consistent with this observation, biochemical assays showed that while TgALD1 was active on F16BP, TgDPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro. Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold.
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Structural and functional divergence of the aldolase fold in Toxoplasma gondii.,Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngo HM, Boulanger MJ J Mol Biol. 2014 Oct 2. pii: S0022-2836(14)00518-X. doi:, 10.1016/j.jmb.2014.09.019. PMID:25284756<ref>PMID:25284756</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Aldolase 3D structures|Aldolase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Deoxyribose-phosphate aldolase]]
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[[Category: Large Structures]]
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[[Category: Anderson, W F]]
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[[Category: Toxoplasma gondii]]
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[[Category: Structural genomic]]
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[[Category: Anderson WF]]
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[[Category: Halavaty, A S]]
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[[Category: Halavaty AS]]
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[[Category: Igarashi, M]]
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[[Category: Igarashi M]]
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[[Category: Minasov, G]]
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[[Category: Minasov G]]
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[[Category: Ngo, H]]
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[[Category: Ngo H]]
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[[Category: Ruan, J]]
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[[Category: Ruan J]]
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[[Category: Shuvalova, L]]
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[[Category: Shuvalova L]]
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[[Category: Ueno, A]]
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[[Category: Ueno A]]
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[[Category: Bradyzoite]]
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[[Category: Brain cyst]]
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[[Category: Chemotherapy]]
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[[Category: Csgid]]
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[[Category: Lyase]]
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[[Category: Tim-barrel]]
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Current revision

1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49

PDB ID 4eiv

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