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4enn

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==Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-carboxymethylguanine==
==Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-carboxymethylguanine==
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<StructureSection load='4enn' size='340' side='right' caption='[[4enn]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
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<StructureSection load='4enn' size='340' side='right'caption='[[4enn]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4enn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ENN FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4enn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ENN FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=C6G:6-(CARBOXYMETHOXY)-9-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-9H-PURIN-2-AMINE'>C6G</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8448&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4enj|4enj]], [[4enk|4enk]], [[4enm|4enm]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C6G:6-(CARBOXYMETHOXY)-9-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-9H-PURIN-2-AMINE'>C6G</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atl1, SPAC1250.04c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2759 Eukaryota])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4enn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4enn OCA], [https://pdbe.org/4enn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4enn RCSB], [https://www.ebi.ac.uk/pdbsum/4enn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4enn ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4enn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4enn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4enn RCSB], [http://www.ebi.ac.uk/pdbsum/4enn PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/ATL1_SCHPO ATL1_SCHPO] Acts as a DNA damage recognition factor. Required for DNA repair from mutagenic O(6)-alkylguanine adducts. Binds O(6)-alkylguanine lesions providing a signal for other DNA repair pathways.
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== Publication Abstract from PubMed ==
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Nucleotide excision repair (NER) has long been known to remove DNA lesions induced by chemical carcinogens, and the molecular mechanism has been partially elucidated. Here we demonstrate that in Schizosaccharomyces pombe a DNA recognition protein, alkyltransferase-like 1 (Atl1), can play a pivotal role in selecting a specific NER pathway, depending on the nature of the DNA modification. The relative ease of dissociation of Atl1 from DNA containing small O(6)-alkylguanines allows accurate completion of global genome repair (GGR), whereas strong Atl1 binding to bulky O(6)-alkylguanines blocks GGR, stalls the transcription machinery, and diverts the damage to transcription-coupled repair. Our findings redraw the initial stages of the NER process in those organisms that express an alkyltransferase-like gene and raise the question of whether or not O(6)-alkylguanine lesions that are poor substrates for the alkyltransferase proteins in higher eukaryotes might, by analogy, signal such lesions for repair by NER.
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Atl1 Regulates Choice between Global Genome and Transcription-Coupled Repair of O(6)-Alkylguanines.,Latypov VF, Tubbs JL, Watson AJ, Marriott AS, McGown G, Thorncroft M, Wilkinson OJ, Senthong P, Butt A, Arvai AS, Millington CL, Povey AC, Williams DM, Santibanez-Koref MF, Tainer JA, Margison GP Mol Cell. 2012 May 31. PMID:22658721<ref>PMID:22658721</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Eukaryota]]
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[[Category: Large Structures]]
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[[Category: Arvai, A S.]]
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[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Tainer, J A.]]
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[[Category: Synthetic construct]]
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[[Category: Tubbs, J L.]]
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[[Category: Arvai AS]]
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[[Category: Alkylation]]
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[[Category: Tainer JA]]
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[[Category: Alkyltransferase]]
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[[Category: Tubbs JL]]
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[[Category: Base repair]]
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[[Category: Dna]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Guanine]]
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[[Category: Ner]]
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[[Category: Nucleotide excision repair]]
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Current revision

Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-carboxymethylguanine

PDB ID 4enn

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