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4exp

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==Structure of mouse Interleukin-34 in complex with mouse FMS==
==Structure of mouse Interleukin-34 in complex with mouse FMS==
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<StructureSection load='4exp' size='340' side='right' caption='[[4exp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<StructureSection load='4exp' size='340' side='right'caption='[[4exp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4exp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EXP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4exp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EXP FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4exn|4exn]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Il34 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Csf1r, Csfmr, Fms ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4exp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exp OCA], [https://pdbe.org/4exp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4exp RCSB], [https://www.ebi.ac.uk/pdbsum/4exp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4exp ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Receptor_protein-tyrosine_kinase Receptor protein-tyrosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 2.7.10.1] </span></td></tr>
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</table>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4exp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4exp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4exp RCSB], [http://www.ebi.ac.uk/pdbsum/4exp PDBsum]</span></td></tr>
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== Function ==
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<table>
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[https://www.uniprot.org/uniprot/IL34_MOUSE IL34_MOUSE] Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Interleukin-34 (IL-34) and colony stimulating factor-1 (CSF-1) both signal through the CSF-1R receptor tyrosine kinase, but they have no sequence homology, and their functions and signaling activities are not identical. We report the crystal structures of mouse IL-34 alone and in complex with the N-terminal three immunoglobulin-like domains (D1-D3) of mouse CSF-1R. IL-34 is structurally related to other helical hematopoietic cytokines, but contains two additional helices integrally associated with the four shared helices. The non-covalently linked IL-34 homodimer recruits two copies of CSF-1R on the sides of the helical bundles, with an overall shape similar to the CSF-1:CSF-1R complex, but the flexible linker between CSF-1R D2 and D3 allows these domains to clamp IL-34 and CSF-1 at different angles. Functional dissection of the IL-34:CSF-1R interface indicates that the hydrophobic interactions, rather than the salt bridge network, dominate the biological activity of IL-34. To degenerately recognize two ligands with completely different surfaces, CSF-1R apparently takes advantage of different subsets of a chemically inert surface that can be tuned to fit different ligand shapes. Differentiated signaling between IL-34 and CSF-1 is likely achieved by the relative thermodynamic independence of IL-34 vs. negative cooperativity of CSF-1 at the receptor-recognition sites, in combination with the difference in hydrophobicity which dictates a more stable IL-34:CSF-1R complex compared to the CSF-1:CSF-1R complex.
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The mechanism of shared but distinct CSF-1R signaling by the non-homologous cytokines IL-34 and CSF-1.,Liu H, Leo C, Chen X, Wong BR, Williams LT, Lin H, He X Biochim Biophys Acta. 2012 May 8. PMID:22579672<ref>PMID:22579672</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Interleukin|Interleukin]]
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*[[Colony-stimulating factor receptor 3D structures|Colony-stimulating factor receptor 3D structures]]
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== References ==
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*[[Interleukin 3D structures|Interleukin 3D structures]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Receptor protein-tyrosine kinase]]
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[[Category: Chen X]]
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[[Category: Chen, X.]]
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[[Category: He X]]
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[[Category: He, X.]]
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[[Category: Leo C]]
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[[Category: Leo, C.]]
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[[Category: Lin H]]
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[[Category: Lin, H.]]
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[[Category: Liu H]]
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[[Category: Liu, H.]]
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[[Category: Williams LT]]
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[[Category: Williams, L T.]]
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[[Category: Wong BR]]
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[[Category: Wong, B R.]]
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[[Category: Beta-sandwich]]
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[[Category: Cytokine-transferase complex]]
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[[Category: Extended 4-helix bundle]]
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[[Category: Immunoglobunin]]
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Current revision

Structure of mouse Interleukin-34 in complex with mouse FMS

PDB ID 4exp

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