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4fmx

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==Crystal Structure of Substrate-Bound P450cin==
==Crystal Structure of Substrate-Bound P450cin==
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<StructureSection load='4fmx' size='340' side='right' caption='[[4fmx]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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<StructureSection load='4fmx' size='340' side='right'caption='[[4fmx]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4fmx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51113 Atcc 51113]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FMX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FMX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4fmx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FMX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.554&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fb2|4fb2]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CIN A, cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fmx OCA], [https://pdbe.org/4fmx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fmx RCSB], [https://www.ebi.ac.uk/pdbsum/4fmx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fmx ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fmx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fmx RCSB], [http://www.ebi.ac.uk/pdbsum/4fmx PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
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[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the P450cin substrate-bound nitric oxide complex and the substrate-free form have been determined. The substrate-free structure adopts an open conformation relative to the substrate-bound structure. The region of the I helix that forms part of the O2 binding pocket shifts from a-helix in the substrate-free form to a p helix in the substrate-bound form. Unique to P450cin is an active site residue, Asn242, in the I helix that H-bonds with the substrate. In most other P450s this residue is Thr which plays an important role in O2 activation by participating in a H-bonding network required for O2 activation. The p/a I helix transition results in the carbonyl O atom of Gly238 moving in to form an H-bond with the water/hydroxide ligand in the substrate-free form. The corresponding residue, Gly248, in P450cam experiences a similar motion and in the oxy-P450cam complex Gly248 adopts a position midway between the substrate-free and -bound states. A comparison between these P450cam and the new P450cin structures provides insights into differences in how the two P450s activate O2. In P450cin, NO and presumably O2, is positioned closer to Gly238 relative to how ligands bind in P450cam. This indicates that Gly238 forms a tighter interaction with diatomic ligands and is the key protein component in the active site that encourages reduction and protonation of the iron-linked O2 by accepting an H-bond from the hydroperoxy intermediate.
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Crystal Structures of Substrate-Free and Nitrosyl Cytochrome P450cin: Implications for O2 Activation.,Madrona Y, Tripathi S, Li H, Poulos TL Biochemistry. 2012 Jul 9. PMID:22775403<ref>PMID:22775403</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 51113]]
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[[Category: Citrobacter braakii]]
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[[Category: Huiying, L]]
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[[Category: Large Structures]]
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[[Category: Madrona, Y]]
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[[Category: Huiying L]]
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[[Category: Poulos, T L]]
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[[Category: Madrona Y]]
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[[Category: Tripathi, S M]]
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[[Category: Poulos TL]]
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[[Category: Cindoxin]]
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[[Category: Tripathi SM]]
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[[Category: Heme]]
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[[Category: Monooxygenase]]
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[[Category: Oxidoreductase]]
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[[Category: P450]]
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Current revision

Crystal Structure of Substrate-Bound P450cin

PDB ID 4fmx

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