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4g2t

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==Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate==
==Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate==
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<StructureSection load='4g2t' size='340' side='right' caption='[[4g2t]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
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<StructureSection load='4g2t' size='340' side='right'caption='[[4g2t]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4g2t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._sf2575 Streptomyces sp. sf2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G2T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4g2t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SF2575 Streptomyces sp. SF2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G2T FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.405&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fzr|4fzr]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g2t OCA], [https://pdbe.org/4g2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g2t RCSB], [https://www.ebi.ac.uk/pdbsum/4g2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g2t ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssfS6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746675 Streptomyces sp. SF2575])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g2t RCSB], [http://www.ebi.ac.uk/pdbsum/4g2t PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/D6MSX4_9ACTN D6MSX4_9ACTN]
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The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 A resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.Proteins 2013. (c) 2013 Wiley Periodicals, Inc.
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Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.,Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584<ref>PMID:23526584</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Streptomyces sp. sf2575]]
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[[Category: Large Structures]]
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[[Category: Bingman, C A]]
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[[Category: Streptomyces sp. SF2575]]
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[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
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[[Category: Bingman CA]]
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[[Category: Phillips, G N]]
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[[Category: Phillips Jr GN]]
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[[Category: Singh, S]]
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[[Category: Singh S]]
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[[Category: Thorson, J S]]
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[[Category: Thorson JS]]
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[[Category: Wang, F]]
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[[Category: Wang F]]
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[[Category: Zhou, M]]
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[[Category: Zhou M]]
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[[Category: Enzyme discovery for natural product biosynthesis]]
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[[Category: Glycosyltransferase]]
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[[Category: Natpro]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Psi-biology]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate

PDB ID 4g2t

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