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4gd0

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{{STRUCTURE_4gd0| PDB=4gd0 | SCENE= }}
 
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===tRNA-guanine transglycosylase Y106F, C158V mutant===
 
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{{ABSTRACT_PUBMED_23704982}}
 
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==Function==
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==tRNA-guanine transglycosylase Y106F, C158V mutant==
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[[http://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO]] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
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<StructureSection load='4gd0' size='340' side='right'caption='[[4gd0]], [[Resolution|resolution]] 1.29&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[4gd0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zymomonas_mobilis_subsp._mobilis_ZM4_=_ATCC_31821 Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GD0 FirstGlance]. <br>
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[[4gd0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zymomonas_mobilis_subsp._mobilis_zm4 Zymomonas mobilis subsp. mobilis zm4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GD0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.29&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gd0 OCA], [https://pdbe.org/4gd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gd0 RCSB], [https://www.ebi.ac.uk/pdbsum/4gd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gd0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TGT_ZYMMO TGT_ZYMMO] Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]
==See Also==
==See Also==
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*[[TRNA-guanine transglycosylase|TRNA-guanine transglycosylase]]
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*[[TRNA-guanine transglycosylase 3D structures|TRNA-guanine transglycosylase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:023704982</ref><references group="xtra"/><references/>
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[[Category: Large Structures]]
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[[Category: Zymomonas mobilis subsp. mobilis zm4]]
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[[Category: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]]
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[[Category: Heine, A.]]
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[[Category: Heine A]]
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[[Category: Klebe, G.]]
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[[Category: Klebe G]]
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[[Category: Reuter, K.]]
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[[Category: Reuter K]]
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[[Category: Schmidt, I.]]
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[[Category: Schmidt I]]
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[[Category: Tidten-Luksch, N.]]
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[[Category: Tidten-Luksch N]]
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[[Category: Bacterial tgt]]
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[[Category: Guanine exchange enzyme]]
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[[Category: Preq1]]
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[[Category: Substrate specificity]]
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[[Category: Transferase]]
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[[Category: Trna]]
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Current revision

tRNA-guanine transglycosylase Y106F, C158V mutant

PDB ID 4gd0

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