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4gq7

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==Crystal structure of Lg-Flo1p==
==Crystal structure of Lg-Flo1p==
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<StructureSection load='4gq7' size='340' side='right' caption='[[4gq7]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
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<StructureSection load='4gq7' size='340' side='right'caption='[[4gq7]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gq7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lager_yeast Lager yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GQ7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gq7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_pastorianus Saccharomyces pastorianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GQ7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.53&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Lg-FLO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=27292 Lager yeast])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gq7 OCA], [http://pdbe.org/4gq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gq7 RCSB], [http://www.ebi.ac.uk/pdbsum/4gq7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gq7 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gq7 OCA], [https://pdbe.org/4gq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gq7 RCSB], [https://www.ebi.ac.uk/pdbsum/4gq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gq7 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B3IUA8_SACPS B3IUA8_SACPS]
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The mechanism of yeast flocculation is generally accepted to be mediated through interaction of cell surface flocculins and mannan carbohydrates. Here, the crystal structure of the soluble 25 kDa lectin domain of Lg-Flo1p flocculin from brewer's yeast was solved to 2.5 A, and its binding specificity towards oligosaccharides investigated by fluorescence spectroscopy. Lg-Flo1p displays broad specificity towards sugars, and interestingly has a 14-fold higher affinity for mannose-1-phosphate and glucose-1-phosphate, compared to their unphosphorylated counterparts. Through structural analysis, we propose that this higher affinity is due to charge interaction with a lysine residue in a carbohydrate binding loop region, NAKAL, unique to NewFlo type flocculins. This raises the possibility of a unique mechanism of flocculation in NewFlo type yeast, which recognizes phosphorylated cell-surface mannans. (c) 2013 Carlsberg Laboratory. Journal compilation (c) 2013 FEBS.
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Structural and biochemical characterization of the N-terminal domain of flocculin, Lg-Flo1p from Saccharomyces pastorianus reveal a unique specificity for phosphorylated mannose.,Sim L, Groes M, Olesen K, Henriksen A FEBS J. 2013 Jan 2. doi: 10.1111/febs.12102. PMID:23281814<ref>PMID:23281814</ref>
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==See Also==
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*[[Flocculation protein 3D structures|Flocculation protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4gq7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lager yeast]]
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[[Category: Large Structures]]
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[[Category: Groes, M]]
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[[Category: Saccharomyces pastorianus]]
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[[Category: Henriksen, A]]
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[[Category: Groes M]]
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[[Category: Olesen, K]]
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[[Category: Henriksen A]]
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[[Category: Sim, L]]
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[[Category: Olesen K]]
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[[Category: Carbohydrate binding domain]]
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[[Category: Sim L]]
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[[Category: Pa14 domain]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of Lg-Flo1p

PDB ID 4gq7

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