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4gvl

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==Crystal Structure of the GsuK RCK domain==
==Crystal Structure of the GsuK RCK domain==
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<StructureSection load='4gvl' size='340' side='right' caption='[[4gvl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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<StructureSection load='4gvl' size='340' side='right'caption='[[4gvl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gvl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens_pca Geobacter sulfurreducens pca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GVL FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gvl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens_PCA Geobacter sulfurreducens PCA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GVL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gx0|4gx0]], [[4gx1|4gx1]], [[4gx2|4gx2]], [[4gx5|4gx5]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GSU0527 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243231 Geobacter sulfurreducens PCA])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gvl OCA], [https://pdbe.org/4gvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gvl RCSB], [https://www.ebi.ac.uk/pdbsum/4gvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gvl ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gvl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gvl RCSB], [http://www.ebi.ac.uk/pdbsum/4gvl PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q74FS9_GEOSL Q74FS9_GEOSL]
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The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K(+) channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K(+) channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca(2+) serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K(+) channels.DOI:http://dx.doi.org/10.7554/eLife.00184.001.
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Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.,Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y elife. 2012;1:e00184. doi: 10.7554/eLife.00184. Epub 2012 Dec 13. PMID:23240087<ref>PMID:23240087</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Geobacter sulfurreducens pca]]
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[[Category: Geobacter sulfurreducens PCA]]
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[[Category: Chen, L]]
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[[Category: Large Structures]]
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[[Category: Derebe, M G]]
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[[Category: Chen L]]
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[[Category: Jiang, Y]]
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[[Category: Derebe MG]]
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[[Category: Kong, C]]
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[[Category: Jiang Y]]
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[[Category: Lam, Y]]
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[[Category: Kong C]]
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[[Category: Sauer, D B]]
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[[Category: Lam Y]]
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[[Category: Ye, S]]
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[[Category: Sauer DB]]
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[[Category: Zeng, W]]
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[[Category: Ye S]]
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[[Category: Adp binding]]
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[[Category: Zeng W]]
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[[Category: Ion channel]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: Nad binding]]
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[[Category: Transport protein]]
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Current revision

Crystal Structure of the GsuK RCK domain

PDB ID 4gvl

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