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3ilf
From Proteopedia
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| - | {{STRUCTURE_3ilf| PDB=3ilf | SCENE= }} | ||
| - | ===Crystal structure of porphyranase A (PorA) in complex with neo-porphyrotetraose=== | ||
| - | {{ABSTRACT_PUBMED_020376150}} | ||
| - | == | + | ==Crystal structure of porphyranase A (PorA) in complex with neo-porphyrotetraose== |
| - | [[3ilf]] is a 1 chain structure with sequence from [ | + | <StructureSection load='3ilf' size='340' side='right'caption='[[3ilf]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | + | == Structural highlights == | |
| - | == | + | <table><tr><td colspan='2'>[[3ilf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ILF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ILF FirstGlance]. <br> |
| - | < | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=L6S:6-O-SULFO-ALPHA-L-GALACTOSE'>L6S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ilf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ilf OCA], [https://pdbe.org/3ilf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ilf RCSB], [https://www.ebi.ac.uk/pdbsum/3ilf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ilf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PORA_ZOBGA PORA_ZOBGA] Cleaves the sulfated polysaccharide porphyran at the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose. Some longer oligosaccharides of even number of residues are also observed. Inactive on the non-sulfated agarose portion of the porphyran backbone. Displays a strict requirement for C6-sulfate in the -2 and +1-binding subsites.<ref>PMID:20376150</ref> <ref>PMID:22778272</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/il/3ilf_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ilf ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Zobellia galactanivorans]] | [[Category: Zobellia galactanivorans]] | ||
| - | [[Category: Barbeyron | + | [[Category: Barbeyron T]] |
| - | [[Category: Correc | + | [[Category: Correc G]] |
| - | [[Category: Czjzek | + | [[Category: Czjzek M]] |
| - | [[Category: Hehemann | + | [[Category: Hehemann JH]] |
| - | [[Category: Helbert | + | [[Category: Helbert W]] |
| - | [[Category: Michel | + | [[Category: Michel G]] |
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Current revision
Crystal structure of porphyranase A (PorA) in complex with neo-porphyrotetraose
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