This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3vv1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:42, 20 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal Structure of Caenorhabditis elegans galectin LEC-6==
==Crystal Structure of Caenorhabditis elegans galectin LEC-6==
-
<StructureSection load='3vv1' size='340' side='right' caption='[[3vv1]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+
<StructureSection load='3vv1' size='340' side='right'caption='[[3vv1]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3vv1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VV1 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3vv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VV1 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lec-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vv1 RCSB], [http://www.ebi.ac.uk/pdbsum/3vv1 PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv1 OCA], [https://pdbe.org/3vv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vv1 RCSB], [https://www.ebi.ac.uk/pdbsum/3vv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vv1 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q9N384_CAEEL Q9N384_CAEEL]
-
Galectins are a group of lectins that can bind carbohydrate chains containing beta-galactoside units. LEC-6, a member of galectins of Caenorhabditis elegans, binds fucose-containing saccharides. We solved the crystal structure of LEC-6 in complex with galactose-beta1,4-fucose (Galbeta1-4Fuc) at 1.5 A resolution. The overall structure of the protein and the identities of the amino-acid residues binding to the disaccharide are similar to those of other galectins. However, further structural analysis and multiple sequence alignment between LEC-6 and other galectins indicate that a glutamic acid residue (Glu67) is important for the preferential binding between LEC-6 and the fucose moiety of the Galbeta1-4Fuc unit. Frontal affinity chromatography analysis indicated that the affinities of E67D and E67A mutants for Galbeta1-4Fuc are lower than that of wild-type LEC-6. Furthermore, the affinities of Glu67 mutants for an endogenous oligosaccharide, which contains a Galbeta1-4Fuc unit, are drastically reduced relative to that of the wild-type protein. We conclude that the Glu67 in the oligosaccharide-binding site assists the recognition of the fucose moiety by LEC-6.
+
-
 
+
-
Structural basis of preferential binding of fucose-containing saccharide by the Caenorhabditis elegans galectin LEC-6.,Makyio H, Takeuchi T, Tamura M, Nishiyama K, Takahashi H, Natsugari H, Arata Y, Kasai KI, Yamada Y, Wakatsuki S, Kato R Glycobiology. 2013 Mar 28. PMID:23481096<ref>PMID:23481096</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
-
[[Category: Arata, Y]]
+
[[Category: Large Structures]]
-
[[Category: Kasai, K]]
+
[[Category: Arata Y]]
-
[[Category: Kato, R]]
+
[[Category: Kasai K]]
-
[[Category: Makyio, H]]
+
[[Category: Kato R]]
-
[[Category: Natsugari, H]]
+
[[Category: Makyio H]]
-
[[Category: Nishiyama, K]]
+
[[Category: Natsugari H]]
-
[[Category: Takahashi, H]]
+
[[Category: Nishiyama K]]
-
[[Category: Takeuchi, T]]
+
[[Category: Takahashi H]]
-
[[Category: Tamura, M]]
+
[[Category: Takeuchi T]]
-
[[Category: Wakatsuki, S]]
+
[[Category: Tamura M]]
-
[[Category: Yamada, Y]]
+
[[Category: Wakatsuki S]]
-
[[Category: 4-fucose binding]]
+
[[Category: Yamada Y]]
-
[[Category: Galactose-1]]
+
-
[[Category: Galectin]]
+
-
[[Category: Sugar binding protein]]
+

Current revision

Crystal Structure of Caenorhabditis elegans galectin LEC-6

PDB ID 3vv1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vv1"
Personal tools