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3vvz

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==Crystal Strucuture of The Rhodamine 6G-Bound Form of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium==
==Crystal Strucuture of The Rhodamine 6G-Bound Form of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium==
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<StructureSection load='3vvz' size='340' side='right' caption='[[3vvz]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
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<StructureSection load='3vvz' size='340' side='right'caption='[[3vvz]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3vvz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salt1 Salt1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VVZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3vvz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._14028S Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VVZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vvx|3vvx]], [[3vvy|3vvy]], [[3vw0|3vw0]], [[3vw1|3vw1]], [[3vw2|3vw2]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RHQ:RHODAMINE+6G'>RHQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STM0580, STM14_0676 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=588858 SALT1])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vvz OCA], [https://pdbe.org/3vvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vvz RCSB], [https://www.ebi.ac.uk/pdbsum/3vvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vvz ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vvz OCA], [http://pdbe.org/3vvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vvz RCSB], [http://www.ebi.ac.uk/pdbsum/3vvz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vvz ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0F6AY66_SALT1 A0A0F6AY66_SALT1]
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RamR is a transcriptional repressor of the gene-encoding RamA protein, which controls the expression of the multidrug efflux system genes acrAB-tolC. RamR is an important multidrug-resistance factor, however, its structure and the identity of the molecules to which it responds have been unknown. Here, we report the crystal structure of RamR in complex with multiple drugs, including berberine, crystal violet, dequalinium, ethidium bromide and rhodamine 6G. All compounds are found to interact with Phe155 of RamR, and each compound is surrounded by different amino acid residues. Binding of these compounds to RamR reduces its DNA-binding affinity, which results in the increased expression of ramA. Our results reveal significant flexibility in the substrate-recognition region of RamR, which regulates the bacterial efflux participating in multidrug resistance.
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The crystal structure of multidrug-resistance regulator RamR with multiple drugs.,Yamasaki S, Nikaido E, Nakashima R, Sakurai K, Fujiwara D, Fujii I, Nishino K Nat Commun. 2013 Jun 26;4:2078. doi: 10.1038/ncomms3078. PMID:23800819<ref>PMID:23800819</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vvz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Salt1]]
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[[Category: Large Structures]]
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[[Category: Nakashima, R]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]]
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[[Category: Nikaido, E]]
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[[Category: Nakashima R]]
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[[Category: Nishino, K]]
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[[Category: Nikaido E]]
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[[Category: Sakurai, K]]
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[[Category: Nishino K]]
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[[Category: Yamaguchi, A]]
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[[Category: Sakurai K]]
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[[Category: Yamasaki, S]]
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[[Category: Yamaguchi A]]
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[[Category: Dna binding]]
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[[Category: Yamasaki S]]
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[[Category: Hth-motif]]
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[[Category: Tetr transcriptional regulator family]]
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[[Category: Transcription regulator]]
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[[Category: Transcriptional regulator]]
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Current revision

Crystal Strucuture of The Rhodamine 6G-Bound Form of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium

PDB ID 3vvz

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