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3w9z

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Crystal structure of DusC

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Retrieved from "http://52.214.119.220/wiki/index.php/3w9z"

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 <StructureSection load='3w9z' size='340' side='right'caption='[[3w9z]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3w9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W9Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3w9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W9Z FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1vhn|1vhn]], [[3b0p|3b0p]], [[3b0u|3b0u]], [[3b0v|3b0v]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b2140, dusC, JW2128, yohI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w9z OCA], [https://pdbe.org/3w9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w9z RCSB], [https://www.ebi.ac.uk/pdbsum/3w9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w9z ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/DUSC_ECOLI DUSC_ECOLI]] Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.
+[https://www.uniprot.org/uniprot/DUSC_ECOLI DUSC_ECOLI] Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Dihydrouridine (D) is one of the most widely conserved tRNA modifications. Dihydrouridine synthase (Dus) is responsible for introducing D modifications into RNA by the reduction of uridine. Recently, a unique substrate-recognition mechanism using a small adapter molecule has been proposed for Thermus thermophilus Dus (TthDusC). To acquire insight regarding its substrate-recognition mechanism, the crystal structure of DusC from Escherichia coli (EcoDusC) was determined at 2.1 A resolution. EcoDusC was shown to be composed of two domains: an N-terminal catalytic domain and a C-terminal tRNA-binding domain. An L-shaped electron density surrounded by highly conserved residues was found in the active site, as observed for TthDus. Structure comparison with TthDus indicated that the N-terminal region has a similar structure, whereas the C-terminal domain has marked differences in its relative orientation to the N-terminal domain as well as in its own structure. These observations suggested that Dus proteins adopt a common substrate-recognition mechanism using an adapter molecule, whereas the manner of tRNA binding is diverse.
-Structure of dihydrouridine synthase C (DusC) from Escherichia coli.,Chen M, Yu J, Tanaka Y, Tanaka M, Tanaka I, Yao M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):834-8. doi:, 10.1107/S1744309113019489. Epub 2013 Jul 27. PMID:23908023<ref>PMID:23908023</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3w9z" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Chen, M]]
+[[Category: Chen M]]
-[[Category: Tanaka, I]]
+[[Category: Tanaka I]]
-[[Category: Tanaka, Y]]
+[[Category: Tanaka Y]]
-[[Category: Yao, M]]
+[[Category: Yao M]]
-[[Category: Yu, J]]
+[[Category: Yu J]]
-[[Category: Oxidoreductase]]
-[[Category: Reductase]]
-[[Category: Tim barrel]]
-[[Category: Trna]]

3w9z

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Crystal structure of DusC

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