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3wd6

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==Crystal structure of Bombyx mori omega-class glutathione transferase in complex with GSH==
==Crystal structure of Bombyx mori omega-class glutathione transferase in complex with GSH==
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<StructureSection load='3wd6' size='340' side='right' caption='[[3wd6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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<StructureSection load='3wd6' size='340' side='right'caption='[[3wd6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3wd6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WD6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3wd6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WD6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wd6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wd6 RCSB], [http://www.ebi.ac.uk/pdbsum/3wd6 PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wd6 OCA], [https://pdbe.org/3wd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wd6 RCSB], [https://www.ebi.ac.uk/pdbsum/3wd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wd6 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A8R5V3_BOMMO A8R5V3_BOMMO]
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Glutathione transferases (GSTs) are major phase II detoxification enzymes that play central roles in the defense against various environmental toxicants as well as oxidative stress. Here we report the crystal structure of an Omega-class glutathione transferase of Bombyx mori, bmGSTO, to gain insight into its catalytic mechanism. The structure of bmGSTO complexed with glutathione determined at a resolution of 2.5A reveals that it exists as a dimer and is structurally similar to Omega-class GSTs with respect to its secondary and tertiary structures. Analysis of a complex between bmGSTO and glutathione showed that bound glutathione was localized to the glutathione-binding site (G-site). Site-directed mutagenesis of bmGSTO mutants indicated that amino acid residues Leu62, Lys65, Lys77, Val78, Glu91 and Ser92 in the G-site contribute to catalytic activity.
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Three-dimensional structure of a Bombyx mori Omega-class glutathione transferase.,Yamamoto K, Suzuki M, Higashiura A, Nakagawa A Biochem Biophys Res Commun. 2013 Sep 6;438(4):588-93. doi:, 10.1016/j.bbrc.2013.08.011. Epub 2013 Aug 11. PMID:23939046<ref>PMID:23939046</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Higashiura, A]]
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[[Category: Bombyx mori]]
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[[Category: Nakagawa, A]]
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[[Category: Large Structures]]
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[[Category: Suzuki, M]]
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[[Category: Higashiura A]]
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[[Category: Yamamoto, K]]
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[[Category: Nakagawa A]]
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[[Category: Electron sharing network]]
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[[Category: Suzuki M]]
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[[Category: Glutathione binding]]
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[[Category: Yamamoto K]]
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[[Category: Transferase]]
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Current revision

Crystal structure of Bombyx mori omega-class glutathione transferase in complex with GSH

PDB ID 3wd6

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