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4ef3

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Current revision (08:49, 20 March 2024) (edit) (undo)
 
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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ef3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EF3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ef3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EF3 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ef3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ef3 OCA], [https://pdbe.org/4ef3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ef3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ef3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ef3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ef3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ef3 OCA], [https://pdbe.org/4ef3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ef3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ef3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ef3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/CUEO_ECOLI CUEO_ECOLI]]
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[https://www.uniprot.org/uniprot/CUEO_ECOLI CUEO_ECOLI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The acetate-bound form of the type II copper was found in the X-ray structure of the multicopper oxidase CueO crystallized in acetate buffer in addition to the conventional OH(-)-bound form as the major resting form. The acetate ion was retained bound to the type II copper even after prolonged exposure of a CueO crystal to X-ray radiation, which led to the stepwise reduction of the Cu centres. However, in this study, when CueO was crystallized in citrate buffer the OH(-)-bound form was present exclusively. This fact shows that an exogenous acetate ion reaches the type II Cu centre through the water channel constructed between domains 1 and 3 in the CueO molecule. It was also found that the enzymatic activity of CueO is enhanced in the presence of acetate ions in the solvent water.
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Exogenous acetate ion reaches the type II copper centre in CueO through the water-excretion channel and potentially affects the enzymatic activity.,Komori H, Kataoka K, Tanaka S, Matsuda N, Higuchi Y, Sakurai T Acta Crystallogr F Struct Biol Commun. 2016 Jul 1;72(Pt 7):558-63. doi:, 10.1107/S2053230X16009237. Epub 2016 Jun 22. PMID:27380373<ref>PMID:27380373</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ef3" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Blue copper oxidase CueO 3D structures|Blue copper oxidase CueO 3D structures]]
*[[Blue copper oxidase CueO 3D structures|Blue copper oxidase CueO 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Current revision

Multicopper Oxidase CueO (Citrate buffer)

PDB ID 4ef3

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