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4k5a

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==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==
==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==
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<StructureSection load='4k5a' size='340' side='right' caption='[[4k5a]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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<StructureSection load='4k5a' size='340' side='right'caption='[[4k5a]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4k5a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K5A FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4k5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K5A FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k5b|4k5b]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5a OCA], [https://pdbe.org/4k5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k5a RCSB], [https://www.ebi.ac.uk/pdbsum/4k5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k5a ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5a RCSB], [http://www.ebi.ac.uk/pdbsum/4k5a PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).
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== Publication Abstract from PubMed ==
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BCL-W is a member of the BCL-2 family of anti-apoptotic proteins. A key event in the regulation of apoptosis is the heterodimerization between anti-apoptotic and pro-apoptotic family members, which involves a conserved surface-exposed groove on the anti-apoptotic proteins. Crystal structures of the ligand binding-competent conformation exist for all anti-apoptotic family members, with the exception of BCL-W, due to the flexibility of the BCL-W groove region. Existing structures had suggested major deviations of the BCL-W groove region from the otherwise structurally highly related remaining anti-apoptotic family members. To capture its ligand binding-competent conformation by counteracting the conformational flexibility of the BCL-W groove, we had selected high-affinity groove-binding designed ankyrin repeat proteins (DARPins) using ribosome-display. We now determined two high-resolution crystal structures of human BCL-W in complex with different DARPins at a resolution of 1.5 and 1.85A, in which the structure of BCL-W is virtually identical, and in both structures BCL-W adopts a conformation extremely similar to the ligand-free conformation of its closest relative BCL-XL. However, distinct differences to all previous BCL-W structures are evident, notably in the ligand-binding region. We provide the first structural explanation for the conformational flexibility of the BCL-W groove region in comparison to other BCL-2 family members. Due to the importance of the anti-apoptotic BCL-2 family as drug targets, the presented crystal structure of ligand binding-competent BCL-W may serve as a valuable basis for structure-based drug design in the future and provides a missing piece for the structural characterization of this protein family.
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Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W.,Schilling J, Schoppe J, Sauer E, Pluckthun A J Mol Biol. 2014 Apr 17. pii: S0022-2836(14)00194-6. doi:, 10.1016/j.jmb.2014.04.010. PMID:24747052<ref>PMID:24747052</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Bos taurus]]
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[[Category: Bovin]]
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[[Category: Escherichia coli]]
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[[Category: Plueckthun, A.]]
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[[Category: Large Structures]]
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[[Category: Sauer, E.]]
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[[Category: Plueckthun A]]
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[[Category: Schilling, J.]]
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[[Category: Sauer E]]
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[[Category: Schoeppe, J.]]
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[[Category: Schilling J]]
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[[Category: Anti-apoptotic bcl-2 family]]
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[[Category: Schoeppe J]]
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[[Category: Apoptosis]]
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[[Category: Bcl-w]]
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[[Category: Crystal structure]]
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[[Category: Darpin]]
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[[Category: Ligand binding-competent conformation]]
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Current revision

Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W

PDB ID 4k5a

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