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4kt6

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==High-resolution crystal structure Streptococcus pyogenes beta-NAD+ glycohydrolase in complex with its endogenous inhibitor IFS reveals a water-rich interface==
==High-resolution crystal structure Streptococcus pyogenes beta-NAD+ glycohydrolase in complex with its endogenous inhibitor IFS reveals a water-rich interface==
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<StructureSection load='4kt6' size='340' side='right' caption='[[4kt6]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
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<StructureSection load='4kt6' size='340' side='right'caption='[[4kt6]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4kt6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strp3 Strp3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KT6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4kt6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS315 Streptococcus pyogenes MGAS315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KT6 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nga ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198466 STRP3]), SpyM3_0129 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198466 STRP3])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt6 OCA], [http://pdbe.org/4kt6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kt6 RCSB], [http://www.ebi.ac.uk/pdbsum/4kt6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt6 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt6 OCA], [https://pdbe.org/4kt6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kt6 RCSB], [https://www.ebi.ac.uk/pdbsum/4kt6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt6 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0H2UTN5_STRP3 A0A0H2UTN5_STRP3]
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One of the virulence factors produced by Streptococcus pyogenes is beta-NAD(+) glycohydrolase (SPN). S. pyogenes injects SPN into the cytosol of an infected host cell using the cytolysin-mediated translocation pathway. As SPN is toxic to bacterial cells themselves, S. pyogenes possesses the ifs gene that encodes an endogenous inhibitor for SPN (IFS). IFS is localized intracellularly and forms a complex with SPN. This intracellular complex must be dissociated during export through the cell envelope. To provide a structural basis for understanding the interactions between SPN and IFS, the complex was overexpressed between the mature SPN (residues 38-451) and the full-length IFS (residues 1-161), but it could not be crystallized. Therefore, limited proteolysis was used to isolate a crystallizable SPNct-IFS complex, which consists of the SPN C-terminal domain (SPNct; residues 193-451) and the full-length IFS. Its crystal structure has been determined by single anomalous diffraction and the model refined at 1.70 A resolution. Interestingly, our high-resolution structure of the complex reveals that the interface between SPNct and IFS is highly rich in water molecules and many of the interactions are water-mediated. The wet interface may facilitate the dissociation of the complex for translocation across the cell envelope.
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High-resolution crystal structure of Streptococcus pyogenes beta-NAD(+) glycohydrolase in complex with its endogenous inhibitor IFS reveals a highly water-rich interface.,Yoon JY, An DR, Yoon HJ, Kim HS, Lee SJ, Im HN, Jang JY, Suh SW J Synchrotron Radiat. 2013 Nov;20(Pt 6):962-7. doi: 10.1107/S0909049513020803., Epub 2013 Sep 29. PMID:24121349<ref>PMID:24121349</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kt6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Strp3]]
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[[Category: Large Structures]]
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[[Category: An, D R]]
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[[Category: Streptococcus pyogenes MGAS315]]
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[[Category: Im, H N]]
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[[Category: An DR]]
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[[Category: Jang, J Y]]
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[[Category: Im HN]]
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[[Category: Kim, H S]]
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[[Category: Jang JY]]
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[[Category: Lee, S J]]
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[[Category: Kim HS]]
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[[Category: Suh, S W]]
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[[Category: Lee SJ]]
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[[Category: Yoon, H J]]
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[[Category: Suh SW]]
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[[Category: Yoon, J Y]]
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[[Category: Yoon H-J]]
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[[Category: Artt motif]]
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[[Category: Yoon JY]]
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[[Category: Beta-nad+ glycohydrolase]]
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[[Category: Hydrolase-hydrolase inhibitor complex]]
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[[Category: Streptococcus pyogene]]
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Current revision

High-resolution crystal structure Streptococcus pyogenes beta-NAD+ glycohydrolase in complex with its endogenous inhibitor IFS reveals a water-rich interface

PDB ID 4kt6

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