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4tm8

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==Crystal structure of 6-phosphogluconolactonase from Mycobacterium smegmatis N131D mutant==
==Crystal structure of 6-phosphogluconolactonase from Mycobacterium smegmatis N131D mutant==
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<StructureSection load='4tm8' size='340' side='right' caption='[[4tm8]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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<StructureSection load='4tm8' size='340' side='right'caption='[[4tm8]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4tm8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TM8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4tm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TM8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tm7|4tm7]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pgl, MSMEG_3099, MSMEI_3021 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tm8 OCA], [https://pdbe.org/4tm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tm8 RCSB], [https://www.ebi.ac.uk/pdbsum/4tm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tm8 ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphogluconolactonase 6-phosphogluconolactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.31 3.1.1.31] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tm8 OCA], [http://pdbe.org/4tm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4tm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tm8 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0QWX6_MYCS2 A0QWX6_MYCS2]
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As an alternative to Darwinian evolution relying on catalytic promiscuity, a protein may acquire auxiliary function upon metal binding, thus providing it with a novel catalytic machinery. Here we show that addition of cupric ions to a 6-phosphogluconolactonase 6-PGLac bearing a putative metal binding site leads to the emergence of peroxidase activity (kcat 7.8 x 10(-2) s(-1), KM 1.1 x 10(-5) M). Both X-ray crystallographic and EPR data of the copper-loaded enzyme Cu.6-PGLac reveal a bis-histidine coordination site, located within a shallow binding pocket capable of accommodating the o-dianisidine substrate.
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Enzyme repurposing of a hydrolase as an emergent peroxidase upon metal binding.,Fujieda N, Schatti J, Stuttfeld E, Ohkubo K, Maier T, Fukuzumi S, Ward TR Chem Sci. 2015 Jul 1;6(7):4060-4065. doi: 10.1039/c5sc01065a. Epub 2015 May 7. PMID:29218172<ref>PMID:29218172</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4tm8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: 6-phosphogluconolactonase]]
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[[Category: Large Structures]]
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[[Category: Mycs2]]
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[[Category: Mycolicibacterium smegmatis MC2 155]]
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[[Category: Fujieda, N]]
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[[Category: Fujieda N]]
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[[Category: Maier, T]]
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[[Category: Maier T]]
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[[Category: Stuttfeld, E]]
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[[Category: Stuttfeld E]]
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[[Category: Hydrolase]]
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[[Category: Rossmann fold]]
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Current revision

Crystal structure of 6-phosphogluconolactonase from Mycobacterium smegmatis N131D mutant

PDB ID 4tm8

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