4u7u
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==Crystal structure of RNA-guided immune Cascade complex from E.coli== |
| + | <StructureSection load='4u7u' size='340' side='right'caption='[[4u7u]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4u7u]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. The January 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cascade and CRISPR'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_1 10.2210/rcsb_pdb/mom_2015_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U7U FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.003Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u7u OCA], [https://pdbe.org/4u7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u7u RCSB], [https://www.ebi.ac.uk/pdbsum/4u7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u7u ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CSE1_ECOLI CSE1_ECOLI] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).<ref>PMID:21255106</ref> <ref>PMID:21460843</ref> <ref>PMID:22621933</ref> <ref>PMID:22521690</ref> A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA.<ref>PMID:21255106</ref> <ref>PMID:21460843</ref> <ref>PMID:22621933</ref> <ref>PMID:22521690</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]] | |
| - | + | *[[CRISPR-Cas|CRISPR-Cas]] | |
| + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cascade and CRISPR]] | ||
| + | [[Category: Escherichia coli K-12]] | ||
| + | [[Category: Escherichia coli str. K-12 substr. MG1655]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: RCSB PDB Molecule of the Month]] | ||
| + | [[Category: Bunkoczi G]] | ||
| + | [[Category: Gong W]] | ||
| + | [[Category: Sheng G]] | ||
| + | [[Category: Wang J]] | ||
| + | [[Category: Wang M]] | ||
| + | [[Category: Wang Y]] | ||
| + | [[Category: Wei Z]] | ||
| + | [[Category: Zhao H]] | ||
Current revision
Crystal structure of RNA-guided immune Cascade complex from E.coli
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