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4xd9

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==Structure of Rpf2-Rrs1 complex involved in ribosome biogenesis==
==Structure of Rpf2-Rrs1 complex involved in ribosome biogenesis==
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<StructureSection load='4xd9' size='340' side='right' caption='[[4xd9]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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<StructureSection load='4xd9' size='340' side='right'caption='[[4xd9]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4xd9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XD9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4xd9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XD9 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xd9 OCA], [http://pdbe.org/4xd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xd9 RCSB], [http://www.ebi.ac.uk/pdbsum/4xd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xd9 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xd9 OCA], [https://pdbe.org/4xd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xd9 RCSB], [https://www.ebi.ac.uk/pdbsum/4xd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xd9 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/C8VMF9_EMENI C8VMF9_EMENI]
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Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. These proteins are necessary for the recruitment of three ribosomal components (5S ribosomal RNA [rRNA], RpL5 and RpL11) to the 90S ribosome precursor and subsequent 27SB pre-rRNA processing. Here we present the crystal structure of the Aspergillus nidulans (An) Rpf2-Rrs1 core complex. The core complex contains the tightly interlocked N-terminal domains of Rpf2 and Rrs1. The Rpf2 N-terminal domain includes a Brix domain characterized by similar N- and C-terminal architecture. The long alpha-helix of Rrs1 joins the C-terminal half of the Brix domain as if it were part of a single molecule. The conserved proline-rich linker connecting the N- and C-terminal domains of Rrs1 wrap around the side of Rpf2 and anchor the C-terminal domain of Rrs1 to a specific site on Rpf2. In addition, gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA. Further analysis of Rpf2-Rrs1 mutants demonstrated that Saccharomyces cerevisiae Rpf2 R236 (corresponds to R238 of AnRpf2) plays a significant role in this binding. Based on these studies and previous reports, we have proposed a model for ribosomal component recruitment to the 90S ribosome precursor.
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Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.,Asano N, Kato K, Nakamura A, Komoda K, Tanaka I, Yao M Nucleic Acids Res. 2015 May 19;43(9):4746-57. doi: 10.1093/nar/gkv305. Epub 2015 , Apr 8. PMID:25855814<ref>PMID:25855814</ref>
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==See Also==
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*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4xd9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Asano, N]]
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[[Category: Aspergillus nidulans FGSC A4]]
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[[Category: Kato, K]]
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[[Category: Large Structures]]
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[[Category: Yao, M]]
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[[Category: Asano N]]
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[[Category: Complex]]
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[[Category: Kato K]]
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[[Category: Translation]]
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[[Category: Yao M]]

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Structure of Rpf2-Rrs1 complex involved in ribosome biogenesis

PDB ID 4xd9

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