This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4xso

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:02, 20 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of apo-form Alr3699/HepE from Anabaena sp. strain PCC 7120==
==Crystal structure of apo-form Alr3699/HepE from Anabaena sp. strain PCC 7120==
-
<StructureSection load='4xso' size='340' side='right' caption='[[4xso]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
+
<StructureSection load='4xso' size='340' side='right'caption='[[4xso]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4xso]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XSO FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4xso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XSO FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xsp|4xsp]], [[4xsr|4xsr]], [[4xsu|4xsu]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xso OCA], [http://pdbe.org/4xso PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xso RCSB], [http://www.ebi.ac.uk/pdbsum/4xso PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xso OCA], [https://pdbe.org/4xso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xso RCSB], [https://www.ebi.ac.uk/pdbsum/4xso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xso ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q8YQW3_NOSS1 Q8YQW3_NOSS1]
-
Formation of the heterocyst envelope polysaccharide (HEP) is a key process for cyanobacterial heterocyst differentiation. The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative proteins termed Alr3698/HepD and Alr3699/HepE. Here we report the crystal structures of HepE in the apo-form and three complex forms that bind to UDP-glucose (UDPG), UDP&amp;glucose and UDP, respectively. The overall structure of HepE displays a typical GT-B fold of glycosyltransferases, comprising two separate beta/alpha/beta Rossmann-fold domains that form an inter-domain substrate-binding crevice. Structural analyses combined with enzymatic assays indicate that HepE is a glucosyltransferase using UDPG as a sugar donor. Further site-directed mutageneses enable us to assign the key residues that stabilize the sugar donor and putative acceptor. Based on the comparative structural analyses, we propose a putative catalytic cycle of HepE, which undergoes "open-closed-open" conformational changes upon binding to the substrates and release of products. These findings provide structural and catalytic insights into the first enzyme involved in the HEP biosynthesis pathway.
+
-
 
+
-
Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis.,Wang XP, Jiang YL, Dai YN, Cheng W, Chen Y, Zhou CZ Glycobiology. 2015 Dec 21. pii: cwv167. PMID:26692049<ref>PMID:26692049</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4xso" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Chen, Y X]]
+
[[Category: Large Structures]]
-
[[Category: Cheng, W]]
+
[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
-
[[Category: Dai, Y N]]
+
[[Category: Chen YX]]
-
[[Category: Jiang, Y L]]
+
[[Category: Cheng W]]
-
[[Category: Wang, X P]]
+
[[Category: Dai YN]]
-
[[Category: Zhou, C Z]]
+
[[Category: Jiang YL]]
-
[[Category: Glycosyltransferase]]
+
[[Category: Wang XP]]
-
[[Category: Gt-b fold]]
+
[[Category: Zhou CZ]]
-
[[Category: Transferase]]
+

Current revision

Crystal structure of apo-form Alr3699/HepE from Anabaena sp. strain PCC 7120

PDB ID 4xso

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xso"
Personal tools