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| | <StructureSection load='4y2k' size='340' side='right'caption='[[4y2k]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4y2k' size='340' side='right'caption='[[4y2k]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4y2k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y2K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4y2k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y2K FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4y2i|4y2i]], [[4y2m|4y2m]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STMDT2_03511 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y2k OCA], [https://pdbe.org/4y2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y2k RCSB], [https://www.ebi.ac.uk/pdbsum/4y2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y2k ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y2k OCA], [http://pdbe.org/4y2k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y2k RCSB], [http://www.ebi.ac.uk/pdbsum/4y2k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y2k ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A140UHB4_SALTM A0A140UHB4_SALTM] |
| - | The coordination bond between gold and sulfur (Au-S) has been widely studied and utilized in many fields. However, detailed investigations on the basic nature of this bond are still lacking. A gold-specific binding protein, GolB, was recently identified, providing a unique opportunity for the study of the Au-S bond at the molecular level. We probed the mechanical strength of the gold-sulfur bond in GolB using single-molecule force spectroscopy. We measured the rupture force of the Au-S bond to be 165 pN, much lower than Au-S bonds measured on different gold surfaces ( approximately 1000 pN). We further solved the structures of apo-GolB and Au(I)-GolB complex using X-ray crystallography. These structures showed that the average Au-S bond length in GolB is much longer than the reported average value of Au-S bonds. Our results highlight the dramatic influence of the unique biological environment on the stability and strength of metal coordination bonds in proteins.
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| - | | + | |
| - | Structural Insights and the Surprisingly Low Mechanical Stability of the Au-S Bond in the Gold-Specific Protein GolB.,Wei W, Sun Y, Zhu M, Liu X, Sun P, Wang F, Gui Q, Meng W, Cao Y, Zhao J J Am Chem Soc. 2015 Dec 16;137(49):15358-61. doi: 10.1021/jacs.5b09895. Epub 2015, Dec 4. PMID:26636614<ref>PMID:26636614</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4y2k" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus typhimurium loeffler 1892]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ma, L]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Wang, F]] | + | [[Category: Ma L]] |
| - | [[Category: Wei, W]] | + | [[Category: Wang F]] |
| - | [[Category: Zhao, J]] | + | [[Category: Wei W]] |
| - | [[Category: Gold binding protein]]
| + | [[Category: Zhao J]] |
| - | [[Category: Metal transport]]
| + | |
| - | [[Category: Redued form]]
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