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| | ==Structure of Cadmium bound KDO8PS from H.pylori== | | ==Structure of Cadmium bound KDO8PS from H.pylori== |
| - | <StructureSection load='4z1c' size='340' side='right' caption='[[4z1c]], [[Resolution|resolution]] 1.93Å' scene=''> | + | <StructureSection load='4z1c' size='340' side='right'caption='[[4z1c]], [[Resolution|resolution]] 1.93Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z1c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z1C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z1C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z1c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z1C FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z1a|4z1a]], [[4z1b|4z1b]], [[4z1d|4z1d]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-8-phosphooctulonate_synthase 3-deoxy-8-phosphooctulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.55 2.5.1.55] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z1c OCA], [https://pdbe.org/4z1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z1c RCSB], [https://www.ebi.ac.uk/pdbsum/4z1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z1c ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z1c OCA], [http://pdbe.org/4z1c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z1c RCSB], [http://www.ebi.ac.uk/pdbsum/4z1c PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/KDSA_HELPY KDSA_HELPY] |
| - | The crystal structure of 3-deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from Helicobacter pylori (HpKDO8PS) was determined alone and within various complexes, revealing an extra helix (HE) that is absent in the structures of KDO8PS from other organisms. In contrast to the metal coordination of the KDO8PS enzyme from Aquifex aeolicus, HpKDO8PS is specifically coordinated with Cd(2+) or Zn(2+) ions, and isothermal titration calorimetry (ITC) and differential scanning fluorimetry (DSF) revealed that Cd(2+) thermally stabilizes the protein structure more efficiently than Zn(2+). In the substrate-bound structure, water molecules play a key role in fixing residues in the proper configuration to achieve a compact structure. Using the structures of HpKDO8PS and API [arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) bisubstrate inhibitor], we generated 21 compounds showing potential HpKDO8PS-binding properties via in silico virtual screening. The capacity of three, avicularin, hyperin, and MC181, to bind to HpKDO8PS was confirmed through saturation transfer difference (STD) experiments, and we identified their specific ligand binding modes by combining competition experiments and docking simulation analysis. Hyperin was confirmed to bind to the A5P binding site, primarily via hydrophilic interaction, whereas MC181 bound to both the PEP and A5P binding sites through hydrophilic and hydrophobic interactions. These results were consistent with the epitope mapping by STD. Our results are expected to provide clues for the development of HpKDO8PS inhibitors.
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| - | Identification of novel scaffolds for potential anti-Helicobacter pylori agents based on the crystal structure of H. pylori 3-deoxy-d-manno-octulosonate 8-phosphate synthase (HpKDO8PS).,Cho S, Im H, Lee KY, Chen J, Kang HJ, Yoon HJ, Min KH, Lee KR, Park HJ, Lee BJ Eur J Med Chem. 2016 Jan 27;108:188-202. doi: 10.1016/j.ejmech.2015.11.036. Epub , 2015 Dec 1. PMID:26649906<ref>PMID:26649906</ref>
| + | ==See Also== |
| - | | + | *[[Kdo-8-phosphate synthase|Kdo-8-phosphate synthase]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4z1c" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 3-deoxy-8-phosphooctulonate synthase]] | + | [[Category: Helicobacter pylori 26695]] |
| - | [[Category: Cho, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Im, H]] | + | [[Category: Cho S]] |
| - | [[Category: Lee, B J]] | + | [[Category: Im H]] |
| - | [[Category: Yoon, H J]]
| + | [[Category: Lee BJ]] |
| - | [[Category: Helicobacter pylori]] | + | [[Category: Yoon HJ]] |
| - | [[Category: Lipopolysaccharide biosynthesis]] | + | |
| - | [[Category: Metal bound]]
| + | |
| - | [[Category: Transferase]]
| + | |
