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| | ==Cellobionic acid phosphorylase - 3-O-beta-D-glucopyranosyl-alpha-D-glucopyranuronic acid complex== | | ==Cellobionic acid phosphorylase - 3-O-beta-D-glucopyranosyl-alpha-D-glucopyranuronic acid complex== |
| - | <StructureSection load='4zli' size='340' side='right' caption='[[4zli]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='4zli' size='340' side='right'caption='[[4zli]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zli]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZLI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZLI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CGC:3-O-BETA-D-GLUCOPYRANOSYL-ALPHA-D-GLUCOPYRANURONIC+ACID'>CGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zle|4zle]], [[4zlf|4zlf]], [[4zlg|4zlg]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellobionic_acid_phosphorylase Cellobionic acid phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.321 2.4.1.321] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zli OCA], [https://pdbe.org/4zli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zli RCSB], [https://www.ebi.ac.uk/pdbsum/4zli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zli ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zli RCSB], [http://www.ebi.ac.uk/pdbsum/4zli PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q21MB1_SACD2 Q21MB1_SACD2] |
| - | The microbial oxidative cellulose degradation system is attracting significant research attention after the recent discovery of lytic polysaccharide mono-oxygenases. A primary product of the oxidative and hydrolytic cellulose degradation system is cellobionic acid (CbA), the aldonic acid form of cellobiose. We previously demonstrated that the intracellular enzyme belonging to glycoside hydrolase (GH) family 94 from cellulolytic fungus and bacterium is cellobionic acid phosphorylase (CBAP), which catalyzes reversible phosphorolysis of CbA into glucose 1-phosphate and gluconic acid (GlcA). In this report we describe the biochemical characterization and the three-dimensional structure of CBAP from the marine cellulolytic bacterium Saccharophagus degradans. Structures of ligand-free and complex forms with CbA, GlcA, and a synthetic disaccharide product from glucuronic acid (GlcUA) were determined at resolutions of up to 1.6 A. The active site is located near the dimer interface. At subsite +1, the carboxylate group of GlcA and CbA is recognized by Arg-609 and Lys-613. Additionally, one residue from the neighboring protomer (Gln-190) is involved in the carboxylate recognition of GlcA. A mutational analysis indicated that these residues are critical for the binding and catalysis of the aldonic and uronic acid acceptors GlcA and GlcUA. Structural and sequence comparisons with other GH94 phosphorylases revealed that CBAPs have a unique subsite +1 with a distinct amino acid residue conservation pattern at this site. This study provides molecular insight into the energetically efficient metabolic pathway of oxidized sugars that links the oxidative cellulolytic pathway to the glycolytic and pentose phosphate pathways in cellulolytic microbes.
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| - | Crystal structure and substrate recognition of cellobionic acid phosphorylase playing a key role in oxidative cellulose degradation by microbes.,Nam YW, Nihira T, Arakawa T, Saito Y, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2015 Jun 3. pii: jbc.M115.664664. PMID:26041776<ref>PMID:26041776</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cellobionic acid phosphorylase]] | + | [[Category: Large Structures]] |
| - | [[Category: Arakawa, T]] | + | [[Category: Saccharophagus degradans 2-40]] |
| - | [[Category: Fushinobu, S]] | + | [[Category: Arakawa T]] |
| - | [[Category: Nam, Y W]] | + | [[Category: Fushinobu S]] |
| - | [[Category: Glycoside hydrolase family 94]] | + | [[Category: Nam YW]] |
| - | [[Category: Intracellular enzyme]]
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| - | [[Category: Oxidative cellulose degradation system]]
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| - | [[Category: Transferase]]
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