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5b0u

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==Crystal structure of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ)==
==Crystal structure of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ)==
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<StructureSection load='5b0u' size='340' side='right' caption='[[5b0u]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
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<StructureSection load='5b0u' size='340' side='right'caption='[[5b0u]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5b0u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B0U FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5b0u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oplophorus_gracilirostris Oplophorus gracilirostris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0U FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0u OCA], [http://pdbe.org/5b0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b0u RCSB], [http://www.ebi.ac.uk/pdbsum/5b0u PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0u OCA], [https://pdbe.org/5b0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0u RCSB], [https://www.ebi.ac.uk/pdbsum/5b0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/LUCI_OPLGR LUCI_OPLGR]] Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.<ref>PMID:10984608</ref>
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[https://www.uniprot.org/uniprot/LUCI_OPLGR LUCI_OPLGR] Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.<ref>PMID:10984608</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19 kDa protein (nanoKAZ) was determined at 1.71 A resolution. The structure consists of 11 antiparallel beta-strands forming a beta-barrel that is capped by 4 short alpha-helices. The structure of nanoKAZ is similar to those of fatty acid-binding proteins (FABPs), even though the amino acid sequence similarity was very low between them. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure.
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Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine.,Tomabechi Y, Hosoya T, Ehara H, Sekine S, Shirouzu M, Inouye S Biochem Biophys Res Commun. 2016 Jan 29;470(1):88-93. doi:, 10.1016/j.bbrc.2015.12.123. Epub 2015 Dec 30. PMID:26746005<ref>PMID:26746005</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Luciferase 3D structures|Luciferase 3D structures]]
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<div class="pdbe-citations 5b0u" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ehara, H]]
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[[Category: Large Structures]]
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[[Category: Sekine, S I]]
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[[Category: Oplophorus gracilirostris]]
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[[Category: Shirouzu, M]]
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[[Category: Ehara H]]
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[[Category: Tomabechi, Y]]
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[[Category: Sekine SI]]
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[[Category: Luminescent protein]]
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[[Category: Shirouzu M]]
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[[Category: Oplophorus luciferase]]
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[[Category: Tomabechi Y]]

Current revision

Crystal structure of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ)

PDB ID 5b0u

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