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5hxl

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==Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus==
==Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus==
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<StructureSection load='5hxl' size='340' side='right' caption='[[5hxl]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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<StructureSection load='5hxl' size='340' side='right'caption='[[5hxl]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5hxl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mago7 Mago7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HXL FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5hxl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_oryzae_70-15 Pyricularia oryzae 70-15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HXL FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hyc|5hyc]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MGG_01005 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=242507 MAGO7])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hxl OCA], [https://pdbe.org/5hxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hxl RCSB], [https://www.ebi.ac.uk/pdbsum/5hxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hxl ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hxl OCA], [http://pdbe.org/5hxl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hxl RCSB], [http://www.ebi.ac.uk/pdbsum/5hxl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hxl ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G4NCW2_MAGO7 G4NCW2_MAGO7]
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Magnaporthe oryzae is a model fungal plant pathogen employed for studying plant-fungi interactions. Whole genome sequencing and bioinformatics analyses revealed that this fungal pathogen has more than 12,000 protein-coding genes with 65% of the genes remaining functionally un-annotated. Here, we determine the structure of the hypothetical protein, MGG_01005 and show that it is the Magnaporthe oryzae Dynein light chain Tctex-type 1 (dynlt1/3), demonstrated by its structural similarity to other orthologous dynlt1 proteins and its conserved interaction with the N-terminus of the Magnaporthe oryzae dynein intermediate chain, MoDyn1I2. In addition, we present the structure of the MGG_01005-MoDyn1I2 complex together with mutagenesis studies that reveals a di-histidine motif interaction with a glutamate residue in the dynein intermediate chain within a conserved molecular interface. These results demonstrate the utility of structure-based annotation and validate it as a viable approach for the molecular assignment of hypothetic proteins from phyto-pathogenic fungi.
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Structure based function-annotation of hypothetical protein MGG_01005 from Magnaporthe oryzae reveals it is the dynein light chain orthologue of dynlt1/3.,Li G, Huang J, Yang J, He D, Wang C, Qi X, Taylor IA, Liu J, Peng YL Sci Rep. 2018 Mar 2;8(1):3952. doi: 10.1038/s41598-018-21667-5. PMID:29500373<ref>PMID:29500373</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5hxl" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Dynein|Dynein]]
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*[[Dynein 3D structures|Dynein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Mago7]]
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[[Category: Large Structures]]
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[[Category: Huang, J]]
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[[Category: Pyricularia oryzae 70-15]]
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[[Category: Li, G]]
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[[Category: Huang J]]
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[[Category: Liu, J]]
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[[Category: Li G]]
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[[Category: Peng, Y]]
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[[Category: Liu J]]
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[[Category: Dynein light chain tctex-1]]
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[[Category: Peng Y]]
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[[Category: Unknown function]]
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Current revision

Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus

PDB ID 5hxl

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