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5jrc

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<StructureSection load='5jrc' size='340' side='right'caption='[[5jrc]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='5jrc' size='340' side='right'caption='[[5jrc]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5jrc]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Microarchaeum_sp._kin4-m Microarchaeum sp. kin4-m]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JRC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JRC FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5jrc]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M] and [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JRC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JRC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jre|5jre]], [[5jr9|5jr9]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NEQ131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228908 Microarchaeum sp. Kin4-M])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jrc OCA], [https://pdbe.org/5jrc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jrc RCSB], [https://www.ebi.ac.uk/pdbsum/5jrc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jrc ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jrc OCA], [http://pdbe.org/5jrc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jrc RCSB], [http://www.ebi.ac.uk/pdbsum/5jrc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jrc ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q74ML9_NANEQ Q74ML9_NANEQ]
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Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced silencing complex, the effector complex of RNA interference (RNAi). Here, we report three crystal structures of Nanoarchaeum equitans (Ne) C3PO. The apo-NeC3PO structure adopts an open form and unravels a potential substrates entryway for the first time. The NeC3PO:ssRNA and NeC3PO:ssDNA complexes fold like closed football with the substrates captured at the inner cavities. The NeC3PO:ssRNA structure represents the only catalytic form C3PO complex available to date; with mutagenesis and in vitro cleavage assays, the structure provides critical insights into the substrate binding and the two-cation-assisted catalytic mechanisms that are shared by eukaryotic C3POs. The work presented here further advances our understanding on the RNAi pathway.
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Structural basis for single-stranded RNA recognition and cleavage by C3PO.,Zhang J, Liu H, Yao Q, Yu X, Chen Y, Cui R, Wu B, Zheng L, Zuo J, Huang Z, Ma J, Gan J Nucleic Acids Res. 2016 Sep 4. pii: gkw776. PMID:27596600<ref>PMID:27596600</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5jrc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Microarchaeum sp. kin4-m]]
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[[Category: Nanoarchaeum equitans Kin4-M]]
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[[Category: Gan, J]]
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[[Category: Unidentified]]
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[[Category: Zhang, J]]
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[[Category: Gan J]]
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[[Category: C3po]]
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[[Category: Zhang J]]
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[[Category: Complex]]
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[[Category: Dna binding protein]]
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Current revision

Crystal structure of NeC3PO in complex with ssRNA.

PDB ID 5jrc

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