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5wtx

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==Structure of Nop9==
==Structure of Nop9==
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<StructureSection load='5wtx' size='340' side='right' caption='[[5wtx]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
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<StructureSection load='5wtx' size='340' side='right'caption='[[5wtx]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5wtx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5wtx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTX FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wty|5wty]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.074&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtx OCA], [http://pdbe.org/5wtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtx RCSB], [http://www.ebi.ac.uk/pdbsum/5wtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtx ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtx OCA], [https://pdbe.org/5wtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wtx RCSB], [https://www.ebi.ac.uk/pdbsum/5wtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST]] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref>
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[https://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The assembly of eukaryotic ribosomes requires numerous factors that transiently associate with evolving pre-ribosomal particles. The Pumilio repeat-containing protein Nop9 briefly associates with the 90S pre-ribosome during its co-transcriptional assembly. Here, we show that Nop9 specifically binds an 11-nucleotide sequence of 18S rRNA that forms the 3' side of the central pseudoknot and helix 28 in the mature subunit. Crystal structures of Nop9 in the free and RNA-bound states reveal a new type of Pumilio repeat protein with a distinct structure, target sequence and RNA-binding mode. Nop9 contains 10 Pumilio repeats arranged into a U-shaped scaffold. The target RNA is recognized by two stretches of repeats in a bipartite manner, and three central bases are unrecognized as a result of the degeneracy of repeats 6 and 7. Our data suggest that Nop9 regulates the folding of 18S rRNA at early assembly stages of 90S.
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Nop9 binds the central pseudoknot region of 18S rRNA.,Wang B, Ye K Nucleic Acids Res. 2017 Jan 3. pii: gkw1323. doi: 10.1093/nar/gkw1323. PMID:28053123<ref>PMID:28053123</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5wtx" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Wang, B]]
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[[Category: Large Structures]]
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[[Category: Ye, K]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Pumilio repeat-containing protein]]
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[[Category: Wang B]]
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[[Category: Rna binding protein]]
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[[Category: Ye K]]
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[[Category: Rna-binding protein]]
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Current revision

Structure of Nop9

PDB ID 5wtx

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