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6n7s

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(New page: '''Unreleased structure''' The entry 6n7s is ON HOLD Authors: Gao, Y., Cui, Y., Zhou, Z., Yang, W. Description: Cryo EM structure of bacteriophage T7 gene product 4 (gp4) helicase prim...)
Current revision (09:20, 20 March 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 6n7s is ON HOLD
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==Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form II)==
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<SX load='6n7s' size='340' side='right' viewer='molstar' caption='[[6n7s]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6n7s]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N7S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7s OCA], [https://pdbe.org/6n7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n7s RCSB], [https://www.ebi.ac.uk/pdbsum/6n7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HELIC_BPT7 HELIC_BPT7] ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination (PubMed:21606333, PubMed:22977246, PubMed:32009150). The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis (PubMed:21606333, PubMed:22977246, PubMed:32009150). ATP or dTTP hydrolysis propels each helicase domain to translocate 2 nt per step sequentially along DNA (PubMed:30679383, PubMed:17604719). Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange (PubMed:9096333, PubMed:8617248). Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present (PubMed:6454135, PubMed:9139692).[HAMAP-Rule:MF_04154]<ref>PMID:17604719</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref> <ref>PMID:30679383</ref> <ref>PMID:32009150</ref> <ref>PMID:6454135</ref> <ref>PMID:8617248</ref> <ref>PMID:9096333</ref> <ref>PMID:9139692</ref>
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Authors: Gao, Y., Cui, Y., Zhou, Z., Yang, W.
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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Description: Cryo EM structure of bacteriophage T7 gene product 4 (gp4) helicase primase DNA complex II
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Gao, Y]]
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__TOC__
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[[Category: Cui, Y]]
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</SX>
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[[Category: Zhou, Z]]
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[[Category: Escherichia phage T7]]
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[[Category: Yang, W]]
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[[Category: Large Structures]]
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[[Category: Cui Y]]
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[[Category: Gao Y]]
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[[Category: Yang W]]
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[[Category: Zhou Z]]

Current revision

Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form II)

6n7s, resolution 4.60Å

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OCA

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